| Detailed Information for Phage Holli |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Sarahanne Davidson |
| Year Found | 2012 |
| Location Found | Corinth, MS USA |
| Finding Institution | University of Alabama at Birmingham |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 34.93N, 88.52W Map |
| Discovery Notes | Sample was collected in a small flower bed approximately 6 inches deep near a dead bush. |
| Naming Notes | The name "Holli" is the middle name of the scientist who discovered the phage. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 1, 2013 |
| Sequencing Facility | Virginia Commonwealth University Nucleic Acids Research Facilities |
| Shotgun Sequencing Method | 454 Pyrosequencing |
| Approximate Shotgun Coverage | 510 |
| Sanger Finishing Reactions | 4 |
| Genome length (bp) | 51162 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGCCGGTAA |
| GC Content | 63.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A4 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are 2mm and slightly hazy. |
| Morphotype | Siphoviridae |
| Number of Genes | 88 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KU985092 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
4.0 x 10^10 |
| Date of SEA Lysate Titering |
Sep 24, 2012 |
| Pitt Freezer Box# |
39 |
| Pitt Freezer Box Grid# |
5 |
| Available Files |
| Final DNAMaster File | Download |