| Detailed Information for Phage Hope4ever |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Brittany Sklenar |
| Year Found | 2012 |
| Location Found | Lawrenceville, GA USA |
| Finding Institution | Georgia Gwinnett College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 33.960305 N, 83.98810 W Map |
| Discovery Notes | I found this phage right out side of my front door of my house. I found this phage in the top soil. I found the phage near an old plant-I bugged some red fire ants. |
| Naming Notes | I choose my name hope4ever because I am going to open a center called Hope Everlasting to help girls someday and Hope means alot to me. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 7, 2013 |
| Sequencing Facility | Virginia Commonwealth University Nucleic Acids Research Facilities |
| Shotgun Sequencing Method | 454 |
| Sequencer Used | 454 Genome Sequencer FLX |
| Approximate Shotgun Coverage | 270 |
| Sanger Finishing Reactions | 4 |
| Genome length (bp) | 50455 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGATGGTAA |
| GC Content | 63.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Queensborough Community College |
| Annotation Status | In GenBank |
| Plaque Notes | my phage looks like a bullseye with a ring of clearness around the cleared away center. |
| Morphotype | Siphoviridae |
| Number of Genes | 88 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH513971 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
35 |
| Pitt Freezer Box Grid# |
51 |
| Available Files |
| Plaque Picture | Download |
| GenBank File for Phamerator | Download |
