Mycobacterium phage IgnatiusPatJac
IgnatiusPatJac EM Picture
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Detailed Information for Phage IgnatiusPatJac
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByOlivia Jacobs
Year Found2020
Location FoundJohannesburg, South Africa
Finding InstitutionUniversity of the Witwatersrand
ProgramUnavailable
From enriched soil sample?No
Isolation Temperature37°C
GPS Coordinates27.98844 S, 26.01014 E Map
Discovery NotesThe mycobacteriophage was extracted from a soil sample found in a residential area that had undergone recent construction. The soil was dug about 15cm below the surface.
Naming NotesThe mycobacteriophage is named after my father, who passed away from cancer in 2020.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedApr 21, 2022
Shotgun Sequencing MethodPacific Biosciences
Approximate Shotgun Coverage45
Genome length (bp)51164
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGACGGTAA
GC Content63.6%
Sequencing NotesSince this phage was sequenced by PacBio, the coverage value refers to coverage level by CCS (Circular Consensus Sequencing) reads rather than raw PacBio reads.
Fasta file available?Yes: Download fasta file
Characterization
ClusterA
SubclusterA1
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUniversity of Pittsburgh
Annotation StatusIn GenBank
Plaque NotesPlaques range in size from 0.5-1.2 cm, are clear, uniformly circular and have defined edges.
MorphotypeSiphoviridae
Number of Genes93
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List
Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionON677304
Refseq NumberNone yet
Published in PaperJacobs, O., et al 2022, Microbiology Resource Announcements: Complete Genome Sequence of Mycobacteriophage IgnatiusPatJac
Archiving Info
Archiving status Not in Pitt Archives
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload