| Detailed Information for Phage Jabith |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | A.J Allen and Jared Bithell |
| Year Found | 2015 |
| Location Found | Crete, NE USA |
| Finding Institution | Doane University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.621004 N, 96.948621 W Map |
| Discovery Notes | It was a 75 degrees F day and found near a pond on Doane campus. |
| Naming Notes | The reverse of AJ's first name and part of Jared's last name. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 29, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1846 |
| Genome length (bp) | 52715 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGTCGGTTA |
| End Determination Notes | at position 27312 bp 39% of reads have and A rather than consensus G |
| GC Content | 63.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A11 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Temperate
Incubation period: 24 hours
HTL titer: 1.3 x 10^10 pfu/ml |
| Morphotype | Siphoviridae |
| Number of Genes | 99 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KY471460 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.3 x 10^10 pfu/ml |
| Date of SEA Lysate Titering |
Nov 30, 2015 |
| Pitt Freezer Box# |
45 |
| Pitt Freezer Box Grid# |
H11 |
| Available Files |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |