| Detailed Information for Phage Jerm |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Mikala Evans & Rohit Jaswaney |
| Year Found | 2011 |
| Location Found | St. Louis, MO USA |
| Finding Institution | Washington University in St. Louis |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 38.647783 N, 90.304917 W Map |
| Discovery Notes | The phage was taken next to Knight's Hall on the Washington University in St. Louis campus. This place had medium foot traffic and was slightly moist. It was taken at 1:07 pm on 9/5/2011. It was 72 degrees outside. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Oct 10, 2013 |
| Sequencing Facility | The McDonnell Genome Institute at Washington University |
| Shotgun Sequencing Method | 454 Pyrosequencing |
| Approximate Shotgun Coverage | 79 |
| Sanger Finishing Reactions | 14 |
| Genome length (bp) | 53163 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGTCGGTTA |
| GC Content | 63.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A2 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques are medium in size and turbid. |
| Morphotype | Siphoviridae |
| Number of Genes | 95 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KX619650 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2011WUSLjermKH |
| SEA Lysate Titer |
5e7 |
| Date of SEA Lysate Titering |
Oct 6, 2011 |
| Pitt Freezer Box# |
31 |
| Pitt Freezer Box Grid# |
55 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |