| Discovery Notes | An alignment of Kalb97 with one of it's closest relatives, Marius, show that Kalb97 has suffered a 630 bp deletion at base 44554. This deletion removes about 266 bp from the 5' end of gene 79 of Marius (Kalb97 gene 76), which is the immunity repressor, and about 10 bp from the 3' end of gene 80 in Marius (Kalb97 gene 77). Therefore the termination site is deleted in Kalb97 gene 77, thus allowing the reading frame to continue. The space for a promoter/regulation and the translation start site for Kalb97 gene 76 as well as about half the N-termal end of the immunity repressor coding region is missing and therefore it is doubtful if this phage has any immunity regulation. The Phagesdb entry for Kalb97 lists it as a Temperate phage but the plaque pictures look to be clear plaques. The deletion is also evident in the NCBI BLAST results where the T:Q From is 90:1 for Marius. |
