Mycobacterium phage Kimona
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| Detailed Information for Phage Kimona | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Kimona Rampersadh |
| Year Found | 2016 |
| Location Found | Durban, South Africa |
| Finding Institution | University of Kwazulu-Natal |
| Program | Mycobacterial Genetics Course, Durban, South Africa |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 30.21467 S, 30.787953 E Map |
| Discovery Notes | This soil sample was collected from a residential area, Umkomaas. The soil was isolated from a garden plot directly in front of a house. The soil sample was taken 4-5cm deep and appeared to be moderately dry. It can be described as dry,dark red soil. |
| Naming Notes | The name of the phage is inspired by the finders First name. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | May 23, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 2244 |
| Genome length (bp) | 50283 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGGCGGTAA |
| GC Content | 64.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | A |
| Subcluster | A19 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members | |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques appeared medium sized and moderately turbid with a "halo". |
| Morphotype | Siphoviridae |
| Number of Genes | 87 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List | |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF472895 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |