Mycobacterium phage Louie6
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Liberone (GI) Neuvillette (FR) FatCactus (A20) Rossetti (L2) RoseMarie (None)Mycobacterium phage Louie6
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| Detailed Information for Phage Louie6 | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Melissa Reyling |
| Year Found | 2012 |
| Location Found | Monroe, WA USA |
| Finding Institution | Washington State University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 47.503971 N, 121.565591 W Map |
| Discovery Notes | The sample was collected outside the city limits of Monroe at the opening of a livestock barn. The sample was collected 1/8th of an inch below the soil surface and the temperature was approximately 21.7 degrees celsius. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 14, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 2247 |
| Genome length (bp) | 50899 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGGTGGTAA |
| GC Content | 64.2% |
| Sequencing Notes | There was 1 deletion present in 10% of this phage population. Deletion 1 is 180 bp long, and is between positions 2329 - 2508, inclusive. At position 3288, 42% of reads have a C rather than consensus T. At position 24258, 25% of reads have a G rather than consensus A. At position 24330, 42% of reads have an A rather than consensus G. At position 25071, 29% of reads have an A rather than consensus G. At position 44550, 32% of reads have a T rather than consensus C. Between 44984-44985, 42% of reads have a T addition. |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | A |
| Subcluster | A3 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members | |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | This phage expresses three sizes of plaques including 1mm,2mm and pin prick. The plaques are circular, turbid and present halos. |
| Morphotype | Siphoviridae |
| Number of Genes | 89 |
| Number of tRNAs | 3 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List | |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KX670788 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Designator | 2012WASUlouie6MLR |
| SEA Lysate Titer | 1.85x10^10 |
| Date of SEA Lysate Titering | Nov 1, 2012 |
| Pitt Freezer Box# | 42 |
| Pitt Freezer Box Grid# | 41 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
