| Detailed Information for Phage Mabel |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | Abel |
| Found By | Andrea Rizzo |
| Year Found | 2016 |
| Location Found | New Haven, CT USA |
| Finding Institution | Southern Connecticut State University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 42°C |
| GPS Coordinates | 41.329162 N, 72.972589 W Map |
| Discovery Notes | Medium moist, dark soil collected from backyard 27-9-2016. Sunny, 23 °C. |
| Naming Notes | The name Abel is a heterograph and a homophone of able; having the necessary power. Mabel rename 2016-12-10. "M" added for it's ability to lyse M. smegmatis. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 9, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Genome length (bp) | 52577 |
| Character of genome ends | Unknown |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGTCGGTTA |
| GC Content | 63.7% |
| Sequencing Notes | between 27176 - 27187 bp consensus contains a string of 10 A's, 30% of reads contain 9 A's |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A11 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Southern Connecticut State University |
| Annotation Status | In GenBank |
| Plaque Notes | Mabel is lytic. Plaque diameter is 4mm. Plaque phenotype according to incubation at 42°C for 48 hours. Of 21 measurable phage, average tail length was determined to be 134.5 nm and average capsid diameter 58.55 nm. |
| Morphotype | Siphoviridae |
| Number of Genes | 99 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN892487 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1e9 |
| Pitt Freezer Box# |
72 |
| Pitt Freezer Box Grid# |
A12 |
| Available Files |
| EM Picture | Download |
| GenBank File for Phamerator | Download |