| Detailed Information for Phage MadMarie |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | BillyBobJoe |
| Found By | Madison Carney and Annmarie Schmid |
| Year Found | 2017 |
| Location Found | North Andover , MA USA |
| Finding Institution | Merrimack College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 42.662 N, 71.125 W Map |
| Discovery Notes | A 50 mL conical tube was obtained and filled with approximately 15 mL of soil from a soil collection site using a spoon. The soil was collected from the dirt under a group of bushes next to the Rogers Center on Merrimack College’s Campus at approximately 1 inch below the surface. The soil collected was damp-dry and contained many root fragments from the bushes |
| Naming Notes | The name was made up and not based on a real person or event. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 25, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1389 |
| Genome length (bp) | 50849 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGGTGGTAA |
| GC Content | 64.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A3 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Merrimack College |
| Annotation Status | In GenBank |
| Plaque Notes | BillyBobJoe was observed to be a temperate lysogenic phage. The plaques were turbid and small in size. |
| Morphotype | Siphoviridae |
| Number of Genes | 87 |
| Number of tRNAs | 3 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH651179 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
74 |
| Pitt Freezer Box Grid# |
A2 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
