| Detailed Information for Phage Makemake |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Julia Dierksheide |
| Year Found | 2014 |
| Location Found | Williams Bay, WI USA |
| Finding Institution | York Community High School |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 42.600595 N, 88.539983 W Map |
| Discovery Notes | Found in composted manure behind a stable. Soil moist. |
| Naming Notes | It was named after the dwarf plant Makemake. It is pronounced Makimaki. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 18, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 2018 |
| Genome length (bp) | 52496 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGATGGTAA |
| GC Content | 63.8% |
| Sequencing Notes | There are multiple mutations noted in this population, consensus sequence is posted. There is some evidence that not all mutations are found in the same particle. At position 5505 bp 27% of reads contain a C rather than consensus T. At position 6823 bp 30% of reads contain a G rather than consensus A. At position 7989 bp 24% of reads contain a G rather than consensus A. At position 10137 bp 23% of reads contain a G rather than consensus A. At position 17813 bp 38% of reads contain a C rather than consensus T. At position 17817 bp 19% of reads contain a C rather than consensus T. At position 18517 bp 21% of reads contain a G rather than consensus A. At position 19051 bp 20% of reads contain a C rather than consensus T. At position 20422 bp 23% of reads contain a G rather than consensus A. At position 25676 bp 28% of reads contain a G rather than consensus A. At position 26558 bp 39% of reads contain an A rather than consensus G. At position 26907 bp 46% of reads contain a C rather than consensus T. At position 32790 bp 18% of reads contain a C rather than consensus T. At position 33351 bp 33% of reads contain a C rather than consensus T. At position 34868 bp 36% of reads contain a C rather than consensus T. At position 35297 bp 23% of reads contain a G rather than consensus A. At position 41094 bp 28% of reads contain a C rather than consensus T. At position 44067 bp 22% of reads contain a C rather than consensus T. At position 47528 bp 30% of reads contain a G rather than consensus A. |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Clear centers and cloudy edges, variable in size, some pinpoint, some larger. |
| Morphotype | Siphoviridae |
| Number of Genes | 93 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KX369584 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
131 |
| Pitt Freezer Box Grid# |
F1 |
| Available Files |
| Plaque Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
