| Detailed Information for Phage MarysWell |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Eduardo Rocha Jr |
| Year Found | 2015 |
| Location Found | Austin, TX USA |
| Finding Institution | St. Edward's University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | Not entered |
| GPS Coordinates | 30.162222 N, 97.808611 W Map |
| Discovery Notes | Founded in Mary Moore Searight Metropolitan Park. Inside an abandoned well that has decomposed objects inside of it. The well is next to a dry river bank and surrounded by a couple dead trees. A few feet from the well is surrounded by live trees |
| Naming Notes | Since it was found inside an abandoned well in Mary Moore Searight Metropolitan Park, the chosen name gives respect to both the park and well which forms the name MarysWell |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 6, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 2194 |
| Genome length (bp) | 51469 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGGAGGTAA |
| GC Content | 60.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A5 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | The plaque forms a clear circle shape in the middle surrounded by a darker shaded circle. This forms the shape of a bulls-eye, which is due to the switch from the lysogenic cycle to the lytic cycle. |
| Morphotype | Siphoviridae |
| Number of Genes | 88 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN586016 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
25 |
| Pitt Freezer Box Grid# |
B9 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
