Mycobacterium phage Methuselah
Methuselah EM Picture
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Detailed Information for Phage Methuselah
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByShelby Clarke
Year Found2010
Location FoundBaltimore, MD USA
Finding InstitutionUniversity of Maryland, Baltimore County
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates39.255466 N, 76.712533 W Map
Discovery NotesDepth was about a quarter of an inch beneath the surface. Temperature was approximately 65°F. The earth was saturated; it had rained recently. The location was beside a pond with algae, many plants, and average foot traffic.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMar 1, 2011
Approximate Shotgun Coverage310
Genome length (bp)50891
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGGTGGTAA
GC Content64.2%
Fasta file available?Yes: Download fasta file
Characterization
ClusterA
SubclusterA3
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesPlaques are approximately 4 mm in diameter and are quite turbid. The phage is lysogenic, and while some plaques vary in size, most are the same size.
MorphotypeSiphoviridae
Number of Genes83
Number of tRNAs3
Number of tmRNAs0
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKC661272
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
SEA Designator 2010UMBCMethuselahSEC
Pitt Freezer Box# 18
Pitt Freezer Box Grid# 15
Available Files
EM PictureDownload