Mycobacterium phage MrGordo
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Detailed Information for Phage MrGordo
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found BySean Kearney
Year Found2010
Location FoundWest Lafayette, IN USA
Finding InstitutionPurdue University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates40.422000 N, 86.916056 W Map
Discovery NotesMrGordo was found about an inch below moist soil in a flower bed. The flower bed was located about ten feet in front of the east entrance of the Agricultural and Biological Engineering building on Purdue's Campus. The temperature on the day of collection was 12.2 C and it was overcast.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 13, 2011
Genome length (bp)50988
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGATGGTAA
GC Content63.8%
Fasta file available?Yes: Download fasta file
Characterization
ClusterA
SubclusterA1
Cluster Life CycleTemperate
Other Cluster Members

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Chargerpower (None)

Abbyshoes (A1)

Abrogate (A1)

Acme (A1)

Adahisdi (A1)

Aeneas (A1)

AFIS (A1)

Agaliana (A1)

Ajay (A1)

Alsfro (A1)

Altman (A1)

Alvin (A1)

Anglerfish (A1)

Applejack (A1)

Arcanine (A1)

Arlo (A1)

Ashballer (A1)

Atkinbua (A1)

BaconJack (A1)

Barriga (A1)

Beatrix (A1)

BeesKnees (A1)

Bethlehem (A1)

Bexan (A1)

Big3 (A1)

Bigfoot (A1)

BigMau (A1)

BigPaolini (A1)

BillKnuckles (A1)

Bircsak (A1)

Blue (A1)

BluSpix (A1)

Bob3 (A1)

Bones (A1)

BPBiebs31 (A1)

Briton15 (A1)

Bruns (A1)

Burton (A1)

Buttons (A1)

Bxb1 (A1)

CactusRose (A1)

Carlyle (A1)

Chanagan (A1)

Ciao (A1)

ConceptII (A1)

Corvo (A1)

Crispicous1 (A1)

Cueylyss (A1)

DD5 (A1)

Dexes (A1)

Doom (A1)

Dreamboat (A1)

DreamCatcher (A1)

DrFeelGood (A1)

Dulcie (A1)

Dussy (A1)

Dynamix (A1)

Edtherson (A1)

Espresso (A1)

Euphoria (A1)

Eyeball (A1)

Fajezeel (A1)

Fascinus (A1)

Fenn (A1)

Forsytheast (A1)

Francis47 (A1)

Froghopper (A1)

Fushigi (A1)

GageAP (A1)

Gandalf20 (A1)

Gompeii16 (A1)

Graduation (A1)

GrecoEtereo (A1)

Greg (A1)

Gwendoluna (A1)

Gyzlar (A1)

Hami1 (A1)

HanShotFirst (A1)

HarryOW (A1)

Hermia (A1)

HermioneGrange (A1)

Homines (A1)

Hope4ever (A1)

Ichabod (A1)

IgnatiusPatJac (A1)

ILeeKay (A1)

Inyanga (A1)

JackSparrow (A1)

Jasper (A1)

JC27 (A1)

Jerm2 (A1)

Jorgensen (A1)

JuliaChild (A1)

Kanely (A1)

KBG (A1)

Kenmech (A1)

Killigrew (A1)

KSSJEB (A1)

Kugel (A1)

Kykar (A1)

KyMonks1A (A1)

Lamina13 (A1)

Lesedi (A1)

Licorice (A1)

LilBib (A1)

Lockley (A1)

Lopton (A1)

Magnar (A1)

Magnito (A1)

Makemake (A1)

Manatee (A1)

Marcell (A1)

Marchy (A1)

Marge (A1)

Maroc7 (A1)

Marsha (A1)

MaryBeth (A1)

McGuire (A1)

MetalQZJ (A1)

Michley (A1)

Mkhuseli (A1)

Molly (A1)

Monet (A1)

Moose (A1)

MPlant7149 (A1)

MrGordo (A1)

Mryolo (A1)

Mule (A1)

Museum (A1)

Naira (A1)

NEHalo (A1)

Nerujay (A1)

Nhonho (A1)

Niza (A1)

Norz (A1)

Ohno789 (A1)

Oogway (A1)

PacerPaul (A1)

Papez (A1)

Paphu (A1)

Paraselene (A1)

Pari (A1)

Parliament (A1)

PascalRango (A1)

PattyP (A1)

Payneful (A1)

Pelly (A1)

Pepe (A1)

Perseus (A1)

Peterson (A1)

Petp2012 (A1)

Petruchio (A1)

PherrisBueller (A1)

PhrostyMug (A1)

PinkPlastic (A1)

Pinto (A1)

Pippin (A1)

Pita2 (A1)

ProMouse (A1)

PSullivan (A1)

QTRlifeCrisis (A1)

Rajelicia (A1)

Rhynn (A1)

RidgeCB (A1)

Ringer (A1)

Rohr (A1)

Rubeus (A1)

Rufus (A1)

Ruotula (A1)

Rutherferd (A1)

Sagefire (A1)

Sandaddy (A1)

Sanya (A1)

SarFire (A1)

Scowl (A1)

Seabiscuit (A1)

Seanderson (A1)

ShortQueendom (A1)

Sibs6 (A1)

SkiPole (A1)

Slagathor (A1)

Smairt (A1)

Smeagol (A1)

Snazzy (A1)

Solon (A1)

Sorpresa (A1)

SpikeBT (A1)

Squee (A1)

StewieG (A1)

STLscum (A1)

StrongArm (A1)

Sumter (A1)

Sunshine924 (A1)

SwissCheese (A1)

Switzer (A1)

Swole (A1)

Target (A1)

Tasp14 (A1)

Teodoridan (A1)

TheloniousMonk (A1)

Thor (A1)

Topgun (A1)

Tote (A1)

Traft412 (A1)

Treddle (A1)

Tripl3t (A1)

Trouble (A1)

Turj99 (A1)

TwoPeat (A1)

U2 (A1)

Violet (A1)

Watermelon (A1)

Wheeler (A1)

Wilkins (A1)

Zeeculate (A1)

Zephyr (A1)

Zeuska (A1)

Chupacabra (A10)

DontArgue (A10)

Drake94 (A10)

DustyMartin (A10)

Eaglepride (A10)

Edison31 (A10)

Goose (A10)

KittenMittens (A10)

Kristoff (A10)

OKCentral2016 (A10)

PeaceMeal1 (A10)

Poompha (A10)

Rebeuca (A10)

RhynO (A10)

Rowdy (A10)

Severus (A10)

Shapes (A10)

Topanga (A10)

Trike (A10)

Twister (A10)

WalterMcMickey (A10)

Aneem (A11)

Bachome (A11)

Bowtie (A11)

Bud (A11)

Ebony (A11)

Et2Brutus (A11)

Fibonacci (A11)

Flaverint (A11)

Gilberta (A11)

Hutc2 (A11)

Insomnia (A11)

Jabith (A11)

Joselito (A11)

Lucivia (A11)

Mabel (A11)

MaCh (A11)

Mulciber (A11)

Munch (A11)

Orange (A11)

Petersenfast (A11)

Salz (A11)

Sham4 (A11)

Snape (A11)

Timothy (A11)

TinyTimmy (A11)

DarthPhader (A12)

Pharaoh (A12)

Refuge (A12)

Steamy (A12)

Zimmer (A12)

Phlei (A13)

Gail (A14)

Jeeves (A14)

Luchador (A14)

SororFago (A14)

Sunhee (A14)

Anaysia (A15)

Anon (A15)

Battleship (A15)

Boohoo (A15)

DekHockey33 (A15)

Epsocamisio (A15)

JSwag (A15)

KatherineG (A15)

LastResort (A15)

Looper (A15)

MinecraftSteve (A15)

Nebulosus (A15)

Nedarya (A15)

Oofda (A15)

ReMo (A15)

Remus (A15)

Rosalind (A15)

ShayRa (A15)

Soups (A15)

Strosahl (A15)

Switzerland (A15)

Waits (A15)

EagleEye (A16)

Lucyedi (A16)

PainterBoy (A16)

40AC (A17)

MyraDee (A18)

Kimona (A19)

20ES (A2)

AbbyPaige (A2)

Acolyte (A2)

Adzzy (A2)

Agape74 (A2)

AN3 (A2)

AN9 (A2)

ANI8 (A2)

AnnaL29 (A2)

Anselm (A2)

ArcherNM (A2)

BabyBack (A2)

Bactobuster (A2)

Baehexic (A2)

BengiVuitton (A2)

Benvolio (A2)

BiancaTri92 (A2)

BobSwaget (A2)

Bradman (A2)

Bugsy (A2)

Caraxes (A2)

Centaur (A2)

Changeling (A2)

Che12 (A2)

ChipMunk (A2)

Colin (A2)

CRB1 (A2)

Crucio (A2)

D29 (A2)

D32 (A2)

Dalmatian (A2)

DBQu4n (A2)

Deloris (A2)

Dignity (A2)

Drake55 (A2)

DudeLittle (A2)

Duplo (A2)

Echild (A2)

Equemioh13 (A2)

EvilGenius (A2)

EZMoney23 (A2)

Fameo (A2)

FiringLine (A2)

First (A2)

Flare16 (A2)

GaugeLDP (A2)

Georgie2 (A2)

HarryHoudini (A2)

Heffalump (A2)

Herbertwm (A2)

IronMan (A2)

Iwokeuplikedis (A2)

Jaan (A2)

Jerm (A2)

JoshKayV (A2)

Journey13 (A2)

Jsquared (A2)

Kalpine (A2)

Kerberos (A2)

KingCyrus (A2)

Koduck (A2)

L5 (A2)

LadyBird (A2)

Landor (A2)

Larenn (A2)

Leogania (A2)

Leviathan (A2)

LilTurb (A2)

Lokk (A2)

Loser (A2)

MajorMajor (A2)

Malec (A2)

MiculUcigas (A2)

Miko (A2)

MissWhite (A2)

Naji (A2)

NaSiaTalie (A2)

NicoleTera (A2)

NothingSpecial (A2)

Odin (A2)

Ph8s (A2)

Phaded (A2)

Piro94 (A2)

Pomar16 (A2)

Power (A2)

Pukovnik (A2)

QueenB2 (A2)

QueenBeesly (A2)

Quokka (A2)

Rachaly (A2)

RedRock (A2)

Retro23 (A2)

SemperFi (A2)

Serenity (A2)

Smeagan (A2)

SnapTap (A2)

StarStuff (A2)

Superchunk (A2)

SweetiePie (A2)

SwirlSquare (A2)

TarsusIV (A2)

TipsytheTRex (A2)

Tomathan (A2)

Travvers (A2)

Trixie (A2)

Trooper (A2)

Turbido (A2)

Updawg (A2)

VA6 (A2)

VC3 (A2)

WeiHuaDA (A2)

Whabigail7 (A2)

WideWale (A2)

XianYue (A2)

Anthony (A20)

Kinmap (A21)

ACFishhook (A3)

Aglet (A3)

AgronaGT15 (A3)

Anubis (A3)

AugsMagnumOpus (A3)

BabyJohn (A3)

BabyRay (A3)

Beauxregard13 (A3)

BlueBird (A3)

BreSam8 (A3)

Bugatti (A3)

BuzzBuzz (A3)

Bxz2 (A3)

Caviar (A3)

Chiqui (A3)

Colbster (A3)

Cullens (A3)

DaHudson (A3)

Daishi (A3)

Dieselweasel (A3)

DropBear (A3)

EpicPhail (A3)

Farber (A3)

Fernando (A3)

Fred313 (A3)

Gemma (A3)

GingkoMaracino (A3)

Giroux (A3)

Grif (A3)

Groupthink (A3)

Grub (A3)

Grum1 (A3)

GtownJaz (A3)

HashRod (A3)

HaveUMetTed (A3)

Heathen (A3)

HelDan (A3)

Heliosoles (A3)

Hercules11 (A3)

Hookmount (A3)

Idleandcovert (A3)

Isca (A3)

JenCasNa (A3)

JeppNRM (A3)

JF2 (A3)

JF4 (A3)

JHC117 (A3)

Jobu08 (A3)

Kachowdy (A3)

KADY (A3)

Kalb97 (A3)

Kalnoky (A3)

Lambert1 (A3)

LarryKay (A3)

LBerry (A3)

Lilith (A3)

Louie6 (A3)

LugYA (A3)

MA5 (A3)

MadMarie (A3)

Mainiac (A3)

Malinsilva (A3)

Manu (A3)

Marchesa (A3)

Margo (A3)

Marie (A3)

Marius (A3)

MarQuardt (A3)

Methuselah (A3)

Microwolf (A3)

Misomonster (A3)

MK4 (A3)

MoneyMay (A3)

MuchMore (A3)

Noella (A3)

Norbert (A3)

OlanP (A3)

Ollie (A3)

P28Green (A3)

Panamaxus (A3)

Pawn (A3)

Pembroke (A3)

Penny1 (A3)

PGHhamlin (A3)

Phantastic (A3)

PhishRPhriends (A3)

Phoebe (A3)

Phoxy (A3)

Phranny (A3)

Pistachio (A3)

Pocahontas (A3)

Popcicle (A3)

PotatoSplit (A3)

Puppy (A3)

PurpleHaze (A3)

QuinnKiro (A3)

Reba (A3)

ResDef (A3)

Rockstar (A3)

Rummer (A3)

Sabia (A3)

Sabinator (A3)

SaturnRing (A3)

Scout (A3)

Snickers (A3)

SoilDragon (A3)

Soshari (A3)

SoYo (A3)

Spike509 (A3)

Stagni (A3)

StepMih (A3)

Taurus (A3)

Texage (A3)

Tiffany (A3)

TNguyen7 (A3)

Todacoro (A3)

Veracruz (A3)

Vix (A3)

Watson (A3)

Wooldri (A3)

Zetzy (A3)

AbbysRanger (A4)

Abdiel (A4)

Achebe (A4)

Albee (A4)

Alberto7 (A4)

Annyong (A4)

Arturo (A4)

AvatarAhPeg (A4)

Avle17 (A4)

Baby16 (A4)

Backyardigan (A4)

Badger (A4)

BangNhom (A4)

Bartimeaus (A4)

BellusTerra (A4)

Blackmoor (A4)

Bombshell (A4)

Broseidon (A4)

Bruiser (A4)

BubbleTrouble (A4)

Bumblebee11 (A4)

Burger (A4)

Caelakin (A4)

Camperdownii (A4)

CentreCat (A4)

Cerulean (A4)

ChampagnePapi (A4)

Chaph (A4)

Cici (A4)

Cindaradix (A4)

Cintron (A4)

Citius (A4)

Clarenza (A4)

Cocoaberry (A4)

Commander (A4)

Connomayer (A4)

Datway (A4)

Deano (A4)

Dhanush (A4)

DirtyDunning (A4)

Druantia (A4)

Eagle (A4)

Eapen (A4)

Eris (A4)

Eros (A4)

Eurydice (A4)

Florean (A4)

Flux (A4)

Funston (A4)

Gadost (A4)

HamSlice (A4)

Happiness (A4)

Holli (A4)

Houdini22 (A4)

Iceman (A4)

ICleared (A4)

Iracema64 (A4)

Jaykayelowell (A4)

JetBlade (A4)

JoongJeon (A4)

Kampy (A4)

Katalie136 (A4)

KFPoly (A4)

Kingmustik0402 (A4)

Koreni (A4)

Kratark (A4)

Kremtemulon (A4)

Kyee (A4)

LappelDuVide (A4)

Lemur (A4)

LeoAvram (A4)

LHTSCC (A4)

LittleB (A4)

LittleGuy (A4)

LochMonster (A4)

Lorenzo (A4)

Lunsford (A4)

Maverick (A4)

Maxo (A4)

Mazhar510 (A4)

Medusa (A4)

MeeZee (A4)

Melvin (A4)

Millski (A4)

Miramae (A4)

Morpher26 (A4)

Morrow (A4)

Mundrea (A4)

Nebs (A4)

Nemo27 (A4)

Noelle (A4)

NorthStar (A4)

NotAPhaseMom (A4)

Nyxis (A4)

Obama12 (A4)

Ohfah (A4)

OKaNui (A4)

Palestino (A4)

Peaches (A4)

Perplexer (A4)

PeterPeter (A4)

PetiteSangsue (A4)

Phacado (A4)

Phelipe (A4)

Phighter1804 (A4)

Phontbonne (A4)

Pipcraft (A4)

Polymorphads (A4)

Pumbaa (A4)

Relief (A4)

Romney (A4)

Roosevelt (A4)

Rosa24 (A4)

Ruin (A4)

Sabertooth (A4)

Scamp (A4)

Shaka (A4)

SheldonCooper (A4)

Shygu2 (A4)

Skipitt (A4)

Sparxx (A4)

Spino (A4)

Stasia (A4)

Stink (A4)

Taquarus (A4)

Thanksgivukkah (A4)

TinaFeyge (A4)

Tinybot (A4)

TiroTheta9 (A4)

TroyPia (A4)

TygerBlood (A4)

Ulysses (A4)

Wander (A4)

Wilbur (A4)

Wile (A4)

Wizard007 (A4)

Xena (A4)

YoSam321 (A4)

AgentM (A5)

Airmid (A5)

Aragog (A5)

Archetta (A5)

Benedict (A5)

Bluefalcon (A5)

Bonamassa (A5)

Chadwick (A5)

Conspiracy (A5)

Coog (A5)

Cuco (A5)

Discoknowium (A5)

Dublin (A5)

ElTiger69 (A5)

ForGetIt (A5)

George (A5)

Ghoulboy (A5)

HuhtaEnerson15 (A5)

Jabiru (A5)

Jovo (A5)

Lev2 (A5)

LittleCherry (A5)

MarysWell (A5)

Micasa (A5)

Midas2 (A5)

Milcery (A5)

Naca (A5)

PetterN (A5)

Phlorence (A5)

PickleBack (A5)

Scorpia (A5)

Swirley (A5)

SydNat (A5)

Tarynearal (A5)

Theia (A5)

Tiger (A5)

UnionJack (A5)

Zolita (A5)

Artemis2UCLA (A6)

BABullseye (A6)

Blinn1 (A6)

Blue7 (A6)

Candra (A6)

Chartreuse (A6)

CloudWang3 (A6)

Cookiedough (A6)

DaVinci (A6)

Dorothea (A6)

EricB (A6)

Garak (A6)

Gladiator (A6)

GreedyLawyer (A6)

Gruunaga (A6)

Hammer (A6)

Helmet (A6)

Hexamo (A6)

Hoot (A6)

Indra (A6)

Isiphiwo (A6)

Jeffabunny (A6)

JewelBug (A6)

Jordennis (A6)

Kazan (A6)

Kipper29 (A6)

Koko (A6)

Lilbunny (A6)

McFly (A6)

Neeharika16 (A6)

Newrala (A6)

Pmask (A6)

Priamo (A6)

Rifter (A6)

Roksolana (A6)

SmellyB (A6)

SuperAwesome (A6)

SuperCallie99 (A6)

ToneTone (A6)

Tucker (A6)

VohminGhazi (A6)

Wiks (A6)

WunderPhul (A6)

Yokurt (A6)

Zaka (A6)

Zulu (A6)

DroogsArmy (A7)

FlyCatcher (A7)

HINdeR (A7)

Sheen (A7)

Timshel (A7)

Toro (A7)

Astro (A8)

Danforth (A8)

Dixon (A8)

Expelliarmus (A8)

Groundhog (A8)

NearlyHeadless (A8)

Phillis (A8)

Roary (A8)

Saintus (A8)

Smeadley (A8)

Stephig9 (A8)

Aliter (A9)

Alma (A9)

Arissanae (A9)

Beemo (A9)

BogosyJay (A9)

Catalina (A9)

Charm (A9)

Conquerage (A9)

Darrell (A9)

DreamTeam1 (A9)

EdogawaKiddo (A9)

Eidsmoe (A9)

Elephantoon (A9)

EmyBug (A9)

ExplosioNervosa (A9)

Fayely (A9)

Hanray (A9)

Holex (A9)

HortumSL17 (A9)

Jiawan (A9)

Keziacharles14 (A9)

Lilleskat (A9)

LoneWolf (A9)

Maminiaina (A9)

Myxus (A9)

Onglai (A9)

PackMan (A9)

Phaeder (A9)

Phonnegut (A9)

Pioneer (A9)

Priya (A9)

Qobbit (A9)

Rahalelujah (A9)

RyeScarlet (A9)

Sachima (A9)

Scherzo (A9)

SheaKeira (A9)

Spouty (A9)

Toaka (A9)

Tubs (A9)

Ugenie5 (A9)

Vanisoa (A9)

Yecey3 (A9)

Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesLarge plaques, approximately 5 mm in diameter. Circular, cloudy edges, clear center - outer ring formation. The outer rings developed more completely about 24 hours after the formation of the plaques. There was a large degree of variability in the plaque sizes (between 2 and 6 mm) and most of the plaques would form with bias towards one side of the plate. The plaques did keep a consistent morphology after the final purification round, but there was still variation in the plaque sizes.
MorphotypeSiphoviridae
Number of Genes92
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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MrGordo_1
(HNH endonuclease)

MrGordo_2

MrGordo_3
(tail protein)

MrGordo_4

MrGordo_5
(putative structural protein)

MrGordo_6
(putative structural protein)

MrGordo_7
(putative structural protein)

MrGordo_8

MrGordo_9
(lysin A)

MrGordo_10
(lysin B)

MrGordo_11
(terminase)

MrGordo_12
(portal protein)

MrGordo_13
(capsid maturation protease)

MrGordo_14
(scaffolding protein)

MrGordo_15
(major capsid protein)

MrGordo_16
(putative structural protein)

MrGordo_17
(head-to-tail adaptor)

MrGordo_18
(head-to-tail stopper)

MrGordo_19

MrGordo_20
(tail terminator)

MrGordo_21
(major tail protein)

MrGordo_22
(tail assembly chaperone)

MrGordo_23
(tail assembly chaperone)

MrGordo_24
(tape measure protein)

MrGordo_25
(putative structural protein)

MrGordo_26

MrGordo_27
(minor tail protein)

MrGordo_28

MrGordo_29

MrGordo_30

MrGordo_31
(putative structural protein)

MrGordo_32

MrGordo_33

MrGordo_34

MrGordo_35
(putative structural protein)

MrGordo_36

MrGordo_37
(integrase)

MrGordo_38

MrGordo_39

MrGordo_40

MrGordo_41

MrGordo_42

MrGordo_43

MrGordo_44
(DNA polymerase I)

MrGordo_45

MrGordo_46

MrGordo_47

MrGordo_48

MrGordo_49

MrGordo_50

MrGordo_51
(phosphoesterase)

MrGordo_52

MrGordo_53

MrGordo_54
(DNA primase)

MrGordo_55
(DNA primase)

MrGordo_56

MrGordo_57

MrGordo_58

MrGordo_59
(EndoVII)

MrGordo_60

MrGordo_61

MrGordo_62
(HNH endonuclease)

MrGordo_63

MrGordo_64
(glutaredoxin)

MrGordo_65
(DnaB-like dsDNA helicase)

MrGordo_66

MrGordo_67

MrGordo_68

MrGordo_69

MrGordo_70
(helix-turn-helix DNA binding domain protein)

MrGordo_71

MrGordo_72
(Cas4 exonuclease)

MrGordo_73

MrGordo_74

MrGordo_75

MrGordo_76
(immunity repressor)

MrGordo_77

MrGordo_78

MrGordo_79

MrGordo_80

MrGordo_81

MrGordo_82

MrGordo_83

MrGordo_84

MrGordo_85

MrGordo_86

MrGordo_87

MrGordo_88
(DNA methyltransferase)

MrGordo_89

MrGordo_90

MrGordo_91

MrGordo_92

Publication Info
Uploaded to GenBank?Yes
GenBank AccessionJN020140
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
SEA Designator 2010PURDmrgordoSMK
SEA Lysate Titer 1.6e9
Date of SEA Lysate Titering Nov 9, 2010
Pitt Freezer Box# 2
Pitt Freezer Box Grid# D10
Available Files
Plaque PictureDownload
EM PictureDownload
Final DNAMaster FileDownload