| Plaque Notes | After 48 hours, both large and small plaques were present in the plates. The large plaques were turbid, irregularly shaped, with a diameter of 2 mm. The small plaques were turbid, regularly/ circular, with a diameter of 1 mm. After five consecutive plaques assays, both large and small plaques were present. Therefore, a parallel plaque assay using a small plaque (1mm in diameter) and a large plaque (2 mm in diameter) was done. The null hypothesis of the parallel plaque assay was that if a small/large plaque is used and large/small plaques only are present in respective plates, then two different phages were present. The alternative hypothesis of the parallel plaque assay was that if a small/large plaque is used and a mixture of large and small plaques are present in respective plates, then the plaques were from a clonal phage population. The results of parallel plaque assay were both large and small plaques, which confirmed the presence of a clonal phage population.
The EM image had a bacteriophage with a long and flexible tail. Therefore, it is a siphoviridae. It has an icosahedral head. Its head to tail ratio is 1:2.7 |
