| Detailed Information for Phage Noxious |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Miriam Klee, Evelyn Godbout |
| Year Found | 2025 |
| Location Found | Orono, ME United States of America |
| Finding Institution | University of Maine, Honors College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 75°C |
| GPS Coordinates | 44.900879 N, 68.659144 W Map |
| Discovery Notes | University of Maine compost facility. Sunny, dry, sampled soil from behind second compost
stall. Sampled by Sally Dixon Molloy |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 25, 2025 |
| Sequencing Facility | Hubbard Center for Genome Studies at the University of New Hampshire |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Paired-end reads |
| Approximate Shotgun Coverage | 754 |
| Genome length (bp) | 49996 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGATGGTAA |
| GC Content | 63.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Maine, Honors College |
| Annotation Status | Being Annotated (Expected completion by 5/1/2026) |
| Plaque Notes | Plaques plated on L-Agar with 7H9 top agar using Mycobacterium Smegmatis as a host produces plaques that range from 0.5mm-7mm in diameter. Half of the diameter in the middle of the plaque is a complete clearing while the other half of the diameter is a turbid ring. Plaques vary greatly in size when they are plated in high density while the size of each plaque becomes more consistent when plated at a lower density, settling at approximately 5mm. |
| Has been Phamerated? | No |
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
