| Detailed Information for Phage PGHhamlin |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Hannah Hamlin |
| Year Found | 2016 |
| Location Found | Allison Park , PA USA |
| Finding Institution | Centre College |
| Program | Kentucky Biomedical Infrastructure Network Small Genomes Discovery Program |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.589722 N, 79.929722 W Map |
| Discovery Notes | The sample came from an area about a foot away from a pond in a rural residential area. The soil was partially frozen and damp. It was about 200 feet away from any houses and trees ranged from 15-25 feet away from the collection sight. |
| Naming Notes | The name comes from the city myself, the founder, is from combined with my last name. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 23, 2016 |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 295 |
| Genome length (bp) | 50840 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGGTGGTAA |
| GC Content | 64.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A3 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Centre College |
| Annotation Status | In GenBank |
| Plaque Notes | My phage forms small, circular, and cloudy plaques. |
| Morphotype | Siphoviridae |
| Number of Genes | 92 |
| Number of tRNAs | 3 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH077583 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
5.2x10^9 pfu/mL |
| Date of SEA Lysate Titering |
Feb 15, 2016 |
| Pitt Freezer Box# |
27 |
| Pitt Freezer Box Grid# |
G6 |
| Available Files |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
