| Detailed Information for Phage Pelly |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Mackenzie Cundiff |
| Year Found | 2022 |
| Location Found | Pellyton, KY United States |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 37.19539 N, 85.09073 W Map |
| Discovery Notes | Pelly phage tends to produces small and large plaques. When a small or large plaque was picked to plate a mixed population was produced either way. |
| Naming Notes | The name for my phage came from the fact that the soil sample was collected from the land my family owns in Pellyton, Kentucky. I wanted something shorter so I named it Pelly. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Apr 11, 2023 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 5009 |
| Genome length (bp) | 53731 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGATGGTAA |
| GC Content | 63.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A1 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | N/A |
| Other Cluster Members |
|
| Annotating Institution | Western Kentucky University |
| Annotation Status | In GenBank |
| Plaque Notes | Large plaques were all the same size and small plaques were all the same size on average. Pelly phage produced clear plaques with very distinct halos.
Phage head averaged 103.94 nm in length.
Phage tail averaged 257.85 nm in length. |
| Morphotype | Siphoviridae |
| Number of Genes | 94 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PQ201081 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.4 x 10^9 |
| Pitt Freezer Box# |
157 |
| Pitt Freezer Box Grid# |
G11 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
