Mycobacterium phage Pipcraft
Pipcraft Plaque Picture
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Detailed Information for Phage Pipcraft
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByJessica Ashcraft and Jacob Robertson
Year Found2013
Location FoundArkadelphia, AR USA
Finding InstitutionOuachita Baptist University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates34.06583 N, 93.11527 W Map
Discovery NotesThe soil was collected on August 24, 2013. It wa taken from a depth of 6.3 centimeters.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 27, 2014
Sequencing FacilityVirginia Commonwealth University Nucleic Acids Research Facilities
Shotgun Sequencing Method454
Sequencer Used454 Genome Sequencer FLX
Approximate Shotgun Coverage98
Sanger Finishing Reactions11
Genome length (bp)51376
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGCCGGTAA
GC Content63.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterA
SubclusterA4
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionOuachita Baptist University
Annotation StatusIn GenBank
Plaque NotesThe plaques form in two different sizes. There is a plaque that is 1mm and a plaque that is 3 mm.
MorphotypeSiphoviridae
Number of Genes85
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List
Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH155875
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
SEA Lysate Titer 4.3x10^9 pfu/mL
Date of SEA Lysate Titering Nov 4, 2013
Pitt Freezer Box# 2
Pitt Freezer Box Grid# E7
Available Files
Plaque PictureDownload
GenBank File for PhameratorDownload