| Detailed Information for Phage Polymorphads |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Adam Krahn |
| Year Found | 2014 |
| Location Found | Holland, MI USA |
| Finding Institution | Hope College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 41.595 N, 86.075 W Map |
| Discovery Notes | Polymorphads was isolated and purified at 32 degrees Celsius from a soil sample taken from a compost patch.
The sample was obtained on August 20, 2014. This phage was discovered in a SEA-PHAGES lab course. |
| Naming Notes | The plaque morphology had a large variation in size. The first part of the name comes from this shift in plaque size from plating to plating. The last three letters of the name represented an abbreviation of my name used by certain members of my family. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 8, 2019 |
| Sequencing Facility | Hope College |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 1095 |
| Genome length (bp) | 51393 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGCCGGTAA |
| GC Content | 63.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A4 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Maine, Honors College |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques were always circular in shape and clear. Their sizes varied significantly. The largest plaque diameter measured was about 3.0mm and the smallest was around 0.2mm. While analyzing growth characteristics it was observed that Polymorphads does not produce plaques when incubated at 42 degrees Celsius. |
| Morphotype | Siphoviridae |
| Number of Genes | 90 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT114158 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
3.6X10^10 pfu/ml |
| Pitt Freezer Box# |
7 |
| Pitt Freezer Box Grid# |
G3 |
| Available Files |
| Plaque Picture | Download |
| GenBank File for Phamerator | Download |
