| Detailed Information for Phage Polyphemus |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Dan Walsh, Stanley Hollinger-Levitsky |
| Year Found | 2024 |
| Location Found | Edinburg, ME United States of America |
| Finding Institution | University of Maine, Honors College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 45.19028 N, 68.62829 W Map |
| Discovery Notes | Soil sample collected from garden bed. |
| Naming Notes | Polyphemus is the one-eyed giant son of Poseidon and Thoosa in Greek mythology, one of the Cyclopes described in Homer's Odyssey. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 4, 2025 |
| Sequencing Facility | Hubbard Center for Genome Studies at the University of New Hampshire |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NovaSeq X Plus |
| Read Type | Single-end reads |
| Read Length | 250 bp |
| Approximate Shotgun Coverage | 36221 |
| Genome length (bp) | 50424 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGTCGGTTA |
| GC Content | 61.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A6 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Maine, Honors College |
| Annotation Status | QC Complete |
| Plaque Notes | Turbid |
| Morphotype | Siphoviridae |
| Number of Genes | 91 |
| Number of tRNAs | 3 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
199 |
| Pitt Freezer Box Grid# |
D2 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
