The Actinobacteriophage Database

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Detailed Information for Phage Pumbaa
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Claire Schaar
Year Found	2013
Location Found	Holland, MI USA
Finding Institution	Hope College
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	Not entered
GPS Coordinates	42.789 N, 86.1 W Map
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Aug 5, 2020
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina
Sequencer Used	Illumina MiSeq
Read Type	Single-end reads
Read Length	150 bp
Approximate Shotgun Coverage	244
Genome length (bp)	51317
Character of genome ends	3' Sticky Overhang
Overhang Length	10 bases
Overhang Sequence	CGGCCGGTAA
GC Content	64.0%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	A
Subcluster	A4
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View Chargerpower (None) Abbyshoes (A1) Abrogate (A1) Acme (A1) Adahisdi (A1) Aeneas (A1) AFIS (A1) Agaliana (A1) Ajay (A1) Alsfro (A1) Altman (A1) Alvin (A1) Anglerfish (A1) Applejack (A1) Arcanine (A1) Arlo (A1) Ashballer (A1) Atkinbua (A1) BaconJack (A1) Barriga (A1) BarrowTuph (A1) Beatrix (A1) BeesKnees (A1) Bethlehem (A1) Bexan (A1) Big3 (A1) Bigchungi (A1) Bigfoot (A1) BigMau (A1) BigPaolini (A1) BillKnuckles (A1) Bircsak (A1) Blue (A1) BluSpix (A1) Bob3 (A1) Bones (A1) BPBiebs31 (A1) Briton15 (A1) Bruns (A1) Burton (A1) Buttons (A1) Bxb1 (A1) CactusRose (A1) Carlyle (A1) Chanagan (A1) Ciao (A1) ConceptII (A1) Corvo (A1) Crispicous1 (A1) Cueylyss (A1) DD5 (A1) Dexes (A1) Doom (A1) Dreamboat (A1) DreamCatcher (A1) DrFeelGood (A1) Dulcie (A1) Dussy (A1) Dynamix (A1) Edtherson (A1) EnzoK (A1) Espresso (A1) Euphoria (A1) Eyeball (A1) Fajezeel (A1) Fascinus (A1) Fenn (A1) Forsytheast (A1) Francis47 (A1) Froghopper (A1) Fushigi (A1) GageAP (A1) Gandalf20 (A1) GMonster (A1) Gompeii16 (A1) Graduation (A1) GrecoEtereo (A1) Greg (A1) Gwendoluna (A1) Gyzlar (A1) Hami1 (A1) HanShotFirst (A1) HarryOW (A1) Hermia (A1) HermioneGrange (A1) Homines (A1) Hope4ever (A1) Ichabod (A1) IgnatiusPatJac (A1) ILeeKay (A1) Inyanga (A1) Iqorha (A1) JackSparrow (A1) Jasper (A1) JC27 (A1) Jerm2 (A1) Jorgensen (A1) JuliaChild (A1) Kanely (A1) KBG (A1) Kenmech (A1) Killigrew (A1) KSSJEB (A1) Kugel (A1) Kykar (A1) KyMonks1A (A1) Lamina13 (A1) Lesedi (A1) Levia (A1) Licorice (A1) LilBib (A1) Lockley (A1) Lopton (A1) LunarLander (A1) Magnar (A1) Magnito (A1) Makemake (A1) Manatee (A1) Marcell (A1) Marchy (A1) Marco3 (A1) Marge (A1) Maroc7 (A1) Marsha (A1) MaryBeth (A1) McGuire (A1) McSinger (A1) MetalQZJ (A1) Michley (A1) Mkhuseli (A1) Molly (A1) Monet (A1) Moose (A1) MPlant7149 (A1) MrGordo (A1) Mryolo (A1) Mule (A1) Museum (A1) Naira (A1) NEHalo (A1) Nerujay (A1) Nhonho (A1) Niza (A1) Norz (A1) Ohno789 (A1) Oogway (A1) PacerPaul (A1) Papez (A1) Paphu (A1) Paraselene (A1) Pari (A1) Parliament (A1) PascalRango (A1) PattyP (A1) Payneful (A1) Pelly (A1) Pepe (A1) Perseus (A1) Peterson (A1) Petp2012 (A1) Petruchio (A1) PherrisBueller (A1) PhineBark (A1) Phlippers (A1) PhrostyMug (A1) PinkPlastic (A1) Pinto (A1) Pippin (A1) Pita2 (A1) ProMouse (A1) PSullivan (A1) QTRlifeCrisis (A1) Raid (A1) Rajelicia (A1) Rhynn (A1) RidgeCB (A1) Ringer (A1) Rohr (A1) Rubeus (A1) Rufus (A1) Ruotula (A1) Rutherferd (A1) Sagefire (A1) Sandaddy (A1) Sanya (A1) SarFire (A1) Scowl (A1) Seabiscuit (A1) Seanderson (A1) ShortQueendom (A1) Sibs6 (A1) SkiPole (A1) Slagathor (A1) Smairt (A1) Smeagol (A1) Snazzy (A1) Solon (A1) Sorpresa (A1) SpikeBT (A1) Squee (A1) StewieG (A1) STLscum (A1) StrongArm (A1) Sumter (A1) Sunshine924 (A1) SwissCheese (A1) Switzer (A1) Swole (A1) Target (A1) Tasp14 (A1) Teodoridan (A1) TheloniousMonk (A1) Thor (A1) Topgun (A1) Tote (A1) Traft412 (A1) Treddle (A1) Tripl3t (A1) Trouble (A1) Turj99 (A1) TwoPeat (A1) U2 (A1) Violet (A1) Watermelon (A1) Wheeler (A1) Wilkins (A1) Zeeculate (A1) Zephyr (A1) Zeuska (A1) Chupacabra (A10) DontArgue (A10) Drake94 (A10) DustyMartin (A10) Eaglepride (A10) Edison31 (A10) Goose (A10) KittenMittens (A10) Kristoff (A10) OKCentral2016 (A10) PeaceMeal1 (A10) Poompha (A10) Rebeuca (A10) RhynO (A10) Rowdy (A10) Severus (A10) Shapes (A10) Topanga (A10) Trike (A10) Twister (A10) WalterMcMickey (A10) Aneem (A11) Bachome (A11) Bowtie (A11) Bud (A11) Ebony (A11) Et2Brutus (A11) Fibonacci (A11) Flaverint (A11) Gilberta (A11) Hutc2 (A11) Insomnia (A11) Jabith (A11) Joselito (A11) Lucivia (A11) Mabel (A11) MaCh (A11) Mulciber (A11) Munch (A11) Orange (A11) Petersenfast (A11) Salz (A11) Sham4 (A11) Snape (A11) Timothy (A11) TinyTimmy (A11) DarthPhader (A12) Pharaoh (A12) Refuge (A12) Steamy (A12) Zimmer (A12) Phlei (A13) Gail (A14) Jeeves (A14) Luchador (A14) SororFago (A14) Sunhee (A14) Anaysia (A15) Anon (A15) Battleship (A15) Boohoo (A15) DekHockey33 (A15) Epsocamisio (A15) JSwag (A15) KatherineG (A15) LastResort (A15) Looper (A15) MinecraftSteve (A15) Nebulosus (A15) Nedarya (A15) Oofda (A15) ReMo (A15) Remus (A15) Rosalind (A15) ShayRa (A15) Soups (A15) Strosahl (A15) Switzerland (A15) Waits (A15) Warrior24 (A15) EagleEye (A16) Lucyedi (A16) PainterBoy (A16) 40AC (A17) MyraDee (A18) Kimona (A19) 20ES (A2) AbbyPaige (A2) Acolyte (A2) Adzzy (A2) Agape74 (A2) AN3 (A2) AN9 (A2) ANI8 (A2) AnnaL29 (A2) Anselm (A2) ArcherNM (A2) BabyBack (A2) Bactobuster (A2) Baehexic (A2) BengiVuitton (A2) Benvolio (A2) BiancaTri92 (A2) BobSwaget (A2) Bradman (A2) Bugsy (A2) Caraxes (A2) Centaur (A2) Changeling (A2) Che12 (A2) ChipMunk (A2) Colin (A2) CRB1 (A2) Crucio (A2) D29 (A2) D32 (A2) Dalmatian (A2) DBQu4n (A2) Deloris (A2) Dignity (A2) Drake55 (A2) DudeLittle (A2) Duplo (A2) Echild (A2) Equemioh13 (A2) EvilGenius (A2) EZMoney23 (A2) Fameo (A2) FiringLine (A2) First (A2) Flare16 (A2) GaugeLDP (A2) Georgie2 (A2) Gratitude (A2) HarryHoudini (A2) Heffalump (A2) Herbertwm (A2) IronMan (A2) Iwokeuplikedis (A2) Jaan (A2) Jerm (A2) JoshKayV (A2) Journey13 (A2) Jsquared (A2) Kalpine (A2) Kerberos (A2) KingCyrus (A2) Koduck (A2) L5 (A2) LadyBird (A2) Landor (A2) Larenn (A2) Leogania (A2) Leviathan (A2) LilTurb (A2) LionsBait (A2) Lokk (A2) Loser (A2) MajorMajor (A2) Malec (A2) MiculUcigas (A2) Miko (A2) MissWhite (A2) Naji (A2) NaSiaTalie (A2) NicoleTera (A2) NothingSpecial (A2) Odin (A2) Ph8s (A2) Phaded (A2) Piro94 (A2) Pomar16 (A2) Power (A2) Pukovnik (A2) QueenB2 (A2) QueenBeesly (A2) Quokka (A2) Rachaly (A2) RedRock (A2) Retro23 (A2) SemperFi (A2) Serenity (A2) Sknot (A2) Smeagan (A2) SnapTap (A2) StarStuff (A2) Superchunk (A2) SweetiePie (A2) SwirlSquare (A2) TarsusIV (A2) Terrific (A2) TipsytheTRex (A2) Tomathan (A2) Travvers (A2) Tristan (A2) Trixie (A2) Trooper (A2) Turbido (A2) Updawg (A2) VA6 (A2) VC3 (A2) WeiHuaDA (A2) Whabigail7 (A2) WideWale (A2) XianYue (A2) Anthony (A20) FatCactus (A20) Kinmap (A21) ACFishhook (A3) Aglet (A3) AgronaGT15 (A3) Anubis (A3) AugsMagnumOpus (A3) BabyJohn (A3) BabyRay (A3) Beauxregard13 (A3) Bigflo (A3) BlueBird (A3) BreSam8 (A3) Bugatti (A3) BuzzBuzz (A3) Bxz2 (A3) Calvinny (A3) Caviar (A3) Chiqui (A3) Colbster (A3) Cullens (A3) DaHudson (A3) Daishi (A3) Dieselweasel (A3) DropBear (A3) EpicPhail (A3) Farber (A3) Fernando (A3) Fred313 (A3) Gemma (A3) GingkoMaracino (A3) Giroux (A3) Grif (A3) Groupthink (A3) Grub (A3) Grum1 (A3) GtownJaz (A3) HashRod (A3) HaveUMetTed (A3) Heathen (A3) HelDan (A3) Heliosoles (A3) Hercules11 (A3) Hookmount (A3) Idleandcovert (A3) Isca (A3) JenCasNa (A3) JeppNRM (A3) JF2 (A3) JF4 (A3) JHC117 (A3) Jobu08 (A3) Kachowdy (A3) KADY (A3) Kalb97 (A3) Kalnoky (A3) Lambert1 (A3) LarryKay (A3) LBerry (A3) Lilith (A3) Louie6 (A3) Lowa (A3) LugYA (A3) MA5 (A3) MadMarie (A3) Mainiac (A3) Malinsilva (A3) Manu (A3) Marchesa (A3) Margo (A3) Marie (A3) Marius (A3) MarQuardt (A3) Methuselah (A3) Microwolf (A3) Misomonster (A3) MK4 (A3) MoneyMay (A3) MuchMore (A3) Noella (A3) Norbert (A3) OlanP (A3) Ollie (A3) P28Green (A3) Panamaxus (A3) Pawn (A3) Pembroke (A3) Penny1 (A3) PGHhamlin (A3) Phantastic (A3) PhishRPhriends (A3) Phoebe (A3) Phoxy (A3) Phranny (A3) Pistachio (A3) Pocahontas (A3) Popcicle (A3) PotatoSplit (A3) Puppy (A3) PurpleHaze (A3) QuinnKiro (A3) Reba (A3) ResDef (A3) Rockstar (A3) Rummer (A3) Sabia (A3) Sabinator (A3) SaturnRing (A3) Scout (A3) Snickers (A3) SoilDragon (A3) Soshari (A3) SoYo (A3) Spike509 (A3) Stagni (A3) StepMih (A3) Taurus (A3) Texage (A3) Tiffany (A3) TinyPebbles (A3) TNguyen7 (A3) Todacoro (A3) Veracruz (A3) Vix (A3) Watson (A3) Wooldri (A3) Zetzy (A3) AbbysRanger (A4) Abdiel (A4) Achebe (A4) Albee (A4) Alberto7 (A4) Annyong (A4) Arturo (A4) AvatarAhPeg (A4) Avle17 (A4) Baby16 (A4) Backyardigan (A4) Badger (A4) BangNhom (A4) Bartimeaus (A4) BellusTerra (A4) Blackmoor (A4) Bombshell (A4) Broseidon (A4) Bruiser (A4) BubbleTrouble (A4) Bumblebee11 (A4) Burger (A4) Caelakin (A4) Camperdownii (A4) CentreCat (A4) Cerulean (A4) ChampagnePapi (A4) Chaph (A4) Cici (A4) Cindaradix (A4) Cintron (A4) Citius (A4) Clarenza (A4) Cocoaberry (A4) Commander (A4) Connomayer (A4) Datway (A4) Deano (A4) Dhanush (A4) DirtyDunning (A4) Druantia (A4) Eagle (A4) Eapen (A4) Eris (A4) Eros (A4) Eurydice (A4) Florean (A4) Flux (A4) Funston (A4) Gadost (A4) HamSlice (A4) Happiness (A4) Holli (A4) Houdini22 (A4) Huxley (A4) Iceman (A4) ICleared (A4) Iracema64 (A4) Jaykayelowell (A4) JetBlade (A4) JoongJeon (A4) Kampy (A4) Katalie136 (A4) KFPoly (A4) Kingmustik0402 (A4) Koan (A4) Koreni (A4) Kratark (A4) Kremtemulon (A4) Kyee (A4) LappelDuVide (A4) Lemur (A4) LeoAvram (A4) LHTSCC (A4) LittleB (A4) LittleGuy (A4) LochMonster (A4) Lorenzo (A4) Lunsford (A4) Maverick (A4) Maxo (A4) Mayonnaise (A4) Mazhar510 (A4) Medusa (A4) MeeZee (A4) Melvin (A4) Millski (A4) Miramae (A4) Morpher26 (A4) Morrow (A4) Mundrea (A4) Nebs (A4) Nemo27 (A4) Noelle (A4) NorthStar (A4) NotAPhaseMom (A4) Nyxis (A4) Obama12 (A4) Ohfah (A4) OKaNui (A4) Palestino (A4) Peaches (A4) Perplexer (A4) PeterPeter (A4) PetiteSangsue (A4) Phacado (A4) Phelipe (A4) Phighter1804 (A4) Phontbonne (A4) Pipcraft (A4) Polymorphads (A4) Pumbaa (A4) Relief (A4) Romney (A4) Roosevelt (A4) Rosa24 (A4) Ruin (A4) Sabertooth (A4) Scamp (A4) SenorClean (A4) Shaka (A4) SheldonCooper (A4) Shygu2 (A4) Skipitt (A4) Sparxx (A4) Spino (A4) Stasia (A4) Stink (A4) Taquarus (A4) Thanksgivukkah (A4) TinaFeyge (A4) Tinybot (A4) TiroTheta9 (A4) TroyPia (A4) TygerBlood (A4) Ulysses (A4) Wander (A4) Wilbur (A4) Wile (A4) Wizard007 (A4) Xena (A4) YoSam321 (A4) AgentM (A5) Airmid (A5) Aragog (A5) Archetta (A5) Benedict (A5) Bluefalcon (A5) Bonamassa (A5) Chadwick (A5) Conspiracy (A5) Coog (A5) Cuco (A5) Discoknowium (A5) Dublin (A5) ElTiger69 (A5) ForGetIt (A5) George (A5) Ghoulboy (A5) HuhtaEnerson15 (A5) Jabiru (A5) Jovo (A5) Lev2 (A5) LittleCherry (A5) MarysWell (A5) Micasa (A5) Midas2 (A5) Milcery (A5) Naca (A5) PetterN (A5) Phlorence (A5) PickleBack (A5) Prinashe11 (A5) Scorpia (A5) Swirley (A5) SydNat (A5) Tarynearal (A5) Theia (A5) Tiger (A5) Twigg (A5) UnionJack (A5) Zolita (A5) Artemis2UCLA (A6) BABullseye (A6) BlessJoy (A6) Blinn1 (A6) Blue7 (A6) Candra (A6) Chartreuse (A6) CloudWang3 (A6) Cookiedough (A6) DaVinci (A6) Dorothea (A6) EricB (A6) Garak (A6) Gladiator (A6) GreedyLawyer (A6) Gruunaga (A6) Hammer (A6) Helmet (A6) Hexamo (A6) Hoot (A6) Indra (A6) Isiphiwo (A6) Jeffabunny (A6) JewelBug (A6) Jordennis (A6) Kazan (A6) Kipper29 (A6) Koko (A6) Lilbunny (A6) McFly (A6) Neeharika16 (A6) Newrala (A6) Pmask (A6) Polyphemus (A6) Priamo (A6) Rifter (A6) Roksolana (A6) SmellyB (A6) SuperAwesome (A6) SuperCallie99 (A6) Temprado (A6) ToneTone (A6) Tucker (A6) VohminGhazi (A6) Wiks (A6) WunderPhul (A6) Yokurt (A6) Zaka (A6) Zulu (A6) DroogsArmy (A7) FlyCatcher (A7) HINdeR (A7) Sheen (A7) Timshel (A7) Toro (A7) Astro (A8) Danforth (A8) Dixon (A8) Expelliarmus (A8) Groundhog (A8) NearlyHeadless (A8) Phillis (A8) Roary (A8) Saintus (A8) Smeadley (A8) Stephig9 (A8) Aliter (A9) Alma (A9) Arissanae (A9) Beemo (A9) BogosyJay (A9) Catalina (A9) Charm (A9) Conquerage (A9) Darrell (A9) DreamTeam1 (A9) EdogawaKiddo (A9) Eidsmoe (A9) Elephantoon (A9) EmyBug (A9) ExplosioNervosa (A9) Fayely (A9) Hanray (A9) Holex (A9) HortumSL17 (A9) Jiawan (A9) Keziacharles14 (A9) Lilleskat (A9) LoneWolf (A9) Maminiaina (A9) Myxus (A9) Onglai (A9) PackMan (A9) Phaeder (A9) Phonnegut (A9) Pioneer (A9) Priya (A9) Qobbit (A9) Rahalelujah (A9) RyeScarlet (A9) Sachima (A9) Scherzo (A9) SheaKeira (A9) Spouty (A9) Toaka (A9) Tubs (A9) Ugenie5 (A9) Vanisoa (A9) Yecey3 (A9)
Annotating Institution	University of Massachusetts Amherst
Annotation Status	In GenBank
Morphotype	Siphoviridae
Number of Genes	89
Number of tRNAs	0
Number of tmRNAs	0
Has been Phamerated?	Yes
Gene List	Click to View Pumbaa_1 (HNH endonuclease) Pumbaa_2 (terminase small subunit) Pumbaa_3 Pumbaa_4 Pumbaa_5 Pumbaa_6 Pumbaa_7 Pumbaa_8 (lysin A) Pumbaa_9 (holin) Pumbaa_10 (lysin B) Pumbaa_11 (terminase large subunit) Pumbaa_12 (portal protein) Pumbaa_13 (capsid maturation protease) Pumbaa_14 (scaffolding protein) Pumbaa_15 (major capsid protein) Pumbaa_16 Pumbaa_17 (head-to-tail adaptor) Pumbaa_18 Pumbaa_19 (head-to-tail stopper) Pumbaa_20 Pumbaa_21 (tail terminator) Pumbaa_22 (major tail protein) Pumbaa_23 (tail assembly chaperone) Pumbaa_24 (tail assembly chaperone) Pumbaa_25 (tape measure protein) Pumbaa_26 (minor tail protein) Pumbaa_27 (minor tail protein) Pumbaa_28 Pumbaa_29 Pumbaa_30 Pumbaa_31 (minor tail protein) Pumbaa_32 Pumbaa_33 (serine integrase) Pumbaa_34 Pumbaa_35 (deoxycytidylate deaminase) Pumbaa_36 Pumbaa_37 Pumbaa_38 Pumbaa_39 Pumbaa_40 (helix-turn-helix DNA binding domain protein) Pumbaa_41 Pumbaa_42 Pumbaa_43 Pumbaa_44 Pumbaa_45 (DNA polymerase I) Pumbaa_46 Pumbaa_47 Pumbaa_48 (ThyX-like thymidylate synthase) Pumbaa_49 Pumbaa_50 (ribonucleotide reductase) Pumbaa_51 (DNA binding protein) Pumbaa_52 (phosphoesterase) Pumbaa_53 Pumbaa_54 Pumbaa_55 (DNA primase) Pumbaa_56 (DNA primase) Pumbaa_57 Pumbaa_58 (endonuclease VII) Pumbaa_59 Pumbaa_60 (hydrolase) Pumbaa_61 (DprA-like DNA processing chain A) Pumbaa_62 Pumbaa_63 (nucleoside deoxyribosyltransferase) Pumbaa_64 (DnaB-like dsDNA helicase) Pumbaa_65 Pumbaa_66 Pumbaa_67 Pumbaa_68 (Cas4 exonuclease) Pumbaa_69 Pumbaa_70 (immunity repressor) Pumbaa_71 Pumbaa_72 Pumbaa_73 Pumbaa_74 Pumbaa_75 Pumbaa_76 Pumbaa_77 Pumbaa_78 (DNA methylase) Pumbaa_79 (DNA methylase) Pumbaa_80 Pumbaa_81 (SprT-like protease) Pumbaa_82 Pumbaa_83 Pumbaa_84 Pumbaa_85 Pumbaa_86 Pumbaa_87 Pumbaa_88 Pumbaa_89
Submitted Annotation in DNA Master Format	Download
Submitted Minimal DNA Master File	Download
Author List	Download
Annotation Cover Sheet	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MZ171380
Refseq Number	None yet
Archiving Info
Archiving status	Archived
SEA Lysate Titer	4.2x10^9
Pitt Freezer Box#	5
Pitt Freezer Box Grid#	B8
Available Files
GenBank File for Phamerator	Download

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