Mycobacterium phage Refuge
Know something about this phage that we don't? Modify its data.
Recently Added Phages
Juji Kangaroo Quibble SeptumDuo FilipManilaRecently Modified Phages
WaddleDee Liltimmytim CosmicLatte Touchdown CatPhinRecently Finished Phages
Liberone (GI) Neuvillette (FR) FatCactus (A20) Rossetti (L2) RoseMarie (None)Mycobacterium phage Refuge
Know something about this phage that we don't? Modify its data.
| Detailed Information for Phage Refuge | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Brittnie Hodsdon, Hateya Levesque, Billy & Jon |
| Year Found | 2016 |
| Location Found | Saint John, ME USA |
| Finding Institution | University of Maine, Fort Kent |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 47.222278 N, 68.761303 W Map |
| Discovery Notes | Discovered near Jalbert crossing along the St. John river. Found in silty sediment, oily sheen and saturated with water. |
| Naming Notes | The name of this refuge, due to inconclusive results from other soil samples. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 6, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1607 |
| Genome length (bp) | 53594 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGGACGTAA |
| GC Content | 63.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | A |
| Subcluster | A12 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members | |
| Annotating Institution | University of Maine, Fort Kent |
| Annotation Status | In GenBank |
| Plaque Notes | Extracted a phage from a soil sample, obtained a single plaque, picked this plaque and did serial dilutions and was able to get a series of plates. Started with web plate and had less plaques with each plate. Amplifid phage stock to get the webbed plate for titering. |
| Morphotype | Siphoviridae |
| Number of Genes | 91 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List | |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK494113 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 4.7 x10^8 pfu/mL |
| Date of SEA Lysate Titering | Sep 1, 2017 |
| Pitt Freezer Box# | 46 |
| Pitt Freezer Box Grid# | D1 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |