Mycobacterium phage Roksolana
Roksolana Plaque Picture
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Detailed Information for Phage Roksolana
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByBachana Billy Bachiashvili
Year Found2020
Location FoundBethlehem, PA USA
Finding InstitutionLehigh University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates40.787571 N, 74.21904 W Map
Discovery Notes*Soil sample collected from an area of mulch in the backyard of a relative's house

*Sample collected on February 6th, 2020, at approx. 9PM

*Sample collected during a period of drizzling rain

*Sample was moist, considering the precipitation occurring, however it was still relatively drier than the surrounding normal, non-mulched soil
Naming NotesAs a Valentine's Day present to a person whom I love very much, I name this phage in honor of her.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 19, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Sequencer UsedIllumina MiSeq
Read TypeSingle-end reads
Read Length150 bp
Approximate Shotgun Coverage1247
Genome length (bp)52702
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGTCGGTTA
GC Content61.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterA
SubclusterA6
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionLehigh University
Annotation StatusIn GenBank
Plaque NotesOn non-webbed plates, plaques are approx. 1.5mm in diameter, much larger than "Pinhole" plaques, and are usually transparent, with minimal clouding. No rings.
MorphotypeSiphoviridae
Number of Genes103
Number of tRNAs3
Number of tmRNAs0
Has been Phamerated?Yes
Gene List
Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMZ456034
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 124
Pitt Freezer Box Grid# H1
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
GenBank File for PhameratorDownload