| Detailed Information for Phage Rufus |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Jordan Grainger & Britt DeVore |
| Year Found | 2011 |
| Location Found | Saint Louis, MO USA |
| Finding Institution | Washington University in St. Louis |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 38.648386 N, 90.310186 W Map |
| Discovery Notes | Depth: 3 cm
Moisture Content: Saturated
Location Description: In a grass field outside Danforth University Center. Soil appears to have a green mold/moss growing on surface. Flies present. Water covers surface. Approximately 7cm from sidewalk. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Oct 10, 2013 |
| Sequencing Facility | The McDonnell Genome Institute at Washington University |
| Shotgun Sequencing Method | 454 |
| Sequencer Used | 454 Genome Sequencer FLX |
| Approximate Shotgun Coverage | 127 |
| Sanger Finishing Reactions | 13 |
| Genome length (bp) | 52357 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGATGGTAA |
| GC Content | 63.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques appear clear and non-turbid, range in diameter from 0.10 cm-0.50 cm, and are circular in morphology. No haloes surround the plaques. |
| Morphotype | Siphoviridae |
| Number of Genes | 93 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KT259047 |
| Refseq Number | NC_028810 |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2011WUSLrufusKH |
| SEA Lysate Titer |
6.6e8 |
| Date of SEA Lysate Titering |
Oct 4, 2011 |
| Pitt Freezer Box# |
31 |
| Pitt Freezer Box Grid# |
49 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |