| Detailed Information for Phage Serenity |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Isabel B. Jones |
| Year Found | 2012 |
| Location Found | Pullman, WA USA |
| Finding Institution | Washington State University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 46.731114 N, 117.179731 W Map |
| Discovery Notes | Found 6 cm from the riverbank of the south fork of the Palouse river in downtown Pullman at a depth of 2 cm.
GPS Coordinates
The temperature was 63degF.
The sample site was very moist(very nearly saturated with water) and contained mostly plant debris, leaves and roots. |
| Naming Notes | Named after the spaceship in the short-lived television series Firefly. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 10, 2013 |
| Sequencing Facility | Virginia Commonwealth University Nucleic Acids Research Facilities |
| Shotgun Sequencing Method | 454 Pyrosequencing |
| Approximate Shotgun Coverage | 340 |
| Genome length (bp) | 52088 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGTCGGTTA |
| GC Content | 62.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A2 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Plaque morphology ranged from 1mm-3mm depending on the concentration of phage particles. The plaques were clear and circular. No special features were noted except the phage propensity towards tiny, almost indistinguishable pinprick plaques and their infuriatingly slow growth rate. |
| Morphotype | Siphoviridae |
| Number of Genes | 97 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KT381276 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2012WASUserenityIBJ |
| SEA Lysate Titer |
3.4 x 10^9 |
| Date of SEA Lysate Titering |
Nov 8, 2012 |
| Pitt Freezer Box# |
42 |
| Pitt Freezer Box Grid# |
46 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
