Mycobacterium phage Sibs6
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Detailed Information for Phage Sibs6
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found BySabrina Tarchione
Year Found2017
Location FoundJohnston, IA USA
Finding InstitutionNorthwestern College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates41.6597 N, 93.7207 W Map
Discovery NotesSoil sample was collected by Courtney Mithelman. The soil was wet and taken slightly below surface level. The temperature at the time of sampling was 48 degrees Fahrenheit and rainy.
Naming NotesThis virus will be named in honor of my family. There are six members of my family and my sibling's names are Isabelle, Sophie, and Benjamin. Their first initial and mine will be combined to created a name. It will be in all capital letter because this is exciting!
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJul 7, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Sequencer UsedIllumina MiSeq
Read TypeSingle-end reads
Read Length150 bp
Approximate Shotgun Coverage1585
Genome length (bp)50210
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGATGGTAA
GC Content63.8%
Sequencing NotesAround position 43620 there is a run of 5 Cs, but in about 41% of reads, there are 6 Cs in this position.
Fasta file available?Yes: Download fasta file
Characterization
ClusterA
SubclusterA1
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionNorthwestern College
Annotation StatusIn GenBank
Plaque NotesMy plaque has an inverse halo. Consisting of a cloudy center and a clear center region. The plaque diameter has ranged in size throughout this process. Variables to credit this are: age of host bacterium, age of agar, agar composition, and time spent in buffer.
MorphotypeSiphoviridae
Number of Genes95
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List
Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH576974
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 72
Pitt Freezer Box Grid# H8
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload