| Detailed Information for Phage SpikeBT |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Brady Holbach, Teddy McNeir |
| Year Found | 2016 |
| Location Found | Kenosha, WI USA |
| Finding Institution | Carthage College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 42.61883 N, 87.82119 W Map |
| Discovery Notes | Taken from soil 3 inches below mulch
Soil was dark and moist |
| Naming Notes | Spike is in reference to the spike at the end of the tail.
B & T are first initials of founder's names |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 24, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 407 |
| Genome length (bp) | 50181 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGATGGTAA |
| GC Content | 63.7% |
| Sequencing Notes | approximately 40% of reads contain a 2245 bp deletion of consensus sequence 26761 - 29005 bp, inclusive
at position 16444 bp approximately 35% of reads contain a C instead of consensus T
|
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Carthage College |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques were large and clear, phage is most likely lytic phage. |
| Morphotype | Siphoviridae |
| Number of Genes | 87 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK359304 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.0 X 10^9 |
| Pitt Freezer Box# |
33 |
| Pitt Freezer Box Grid# |
D1 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
