Mycobacterium phage Squee
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| Detailed Information for Phage Squee | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Jamie le Roux |
| Year Found | 2019 |
| Location Found | Auckland, New Zealand |
| Finding Institution | Massey University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 37°C |
| GPS Coordinates | 36.790857 S, 174.65929 E Map |
| Discovery Notes | Found in compost bin 3.2 at Hobsinville Point community garden. Found by a lab mate, Danielle Williams, and adopted by me, Jamie le Roux. |
| Naming Notes | Squee is a word used to express delight or excitment - something I definitely feel about phage discovery! |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jun 26, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 378 |
| Genome length (bp) | 52551 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGATGGTAA |
| GC Content | 63.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | A |
| Subcluster | A1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members | |
| Annotating Institution | Massey University |
| Annotation Status | In GenBank |
| Plaque Notes | Clear, circular, diameter of 30mm. |
| Morphotype | Siphoviridae |
| Number of Genes | 92 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List | |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PP405135 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 93 |
| Pitt Freezer Box Grid# | G3 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
