| Detailed Information for Phage TipsytheTRex |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | TipsytheTRex |
| Found By | Brooke Burns |
| Year Found | 2015 |
| Location Found | Cullowhee, NC USA |
| Finding Institution | Western Carolina University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 24°C |
| GPS Coordinates | 35.3121 N, 83.1805 W Map |
| Discovery Notes | Tipsy was discovered on Western Carolina University's campus between the Stillwell Science building and McKee building near a drain. The soil sample had a worm in it. |
| Naming Notes | TipsytheTRex originated due to the phage's large capsid, which may cause the phage to be 'tipsy' because it has a small tail to support it, similar to that of a T-Rex. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Mar 1, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1969 |
| Genome length (bp) | 52350 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGTCGGTTA |
| GC Content | 62.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A2 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | Since it is a temperate phage, mesas have grown from it. |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Tipsy is a temperate phage with clear, large plaques. |
| Morphotype | Siphoviridae |
| Number of Genes | 93 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF919536 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
3.2 x 10^10 |
| Pitt Freezer Box# |
19 |
| Pitt Freezer Box Grid# |
H3 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
