| Detailed Information for Phage ToneTone |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Madison Martin, Eric Burn, and Mackenzie Herman |
| Year Found | 2014 |
| Location Found | Boulder, CO USA |
| Finding Institution | University of Colorado Boulder |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.0098 N, 105.27 W Map |
| Discovery Notes | Garden south of recreational center
overlooking pool. Damp from sprinklers.
9/10/14
11:00 am, 25ºC |
| Naming Notes | Named after Eric Burn’s high-school biology teacher, Jennifer Toner |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 22, 2014 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 400 |
| Genome length (bp) | 52044 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGTCGGTAA |
| GC Content | 61.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A6 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are approximately 3 mm in diameter
and turbid
Incubated for 48 hours at 37ºC |
| Morphotype | Siphoviridae |
| Number of Genes | 96 |
| Number of tRNAs | 3 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KX375815 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
4X10e10 |
| Date of SEA Lysate Titering |
Oct 17, 2014 |
| Pitt Freezer Box# |
9 |
| Pitt Freezer Box Grid# |
C2 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
