| Detailed Information for Phage Treddle |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | April Burch |
| Year Found | 2012 |
| Location Found | Mill River, MA USA |
| Finding Institution | Berkshire School |
| Program | Phage Hunters Integrating Research and Education |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 42.011162 N, 73.275016 W Map |
| Discovery Notes | Isolated from a vegetable garden that contained soil from Laurel Brook Dairy Farm (390 Norfolk Rd, East Canaan, CT). Soil was 40% compost. |
| Naming Notes | Treddle is a nickname for a close friend of mine. It is also is a term for a strumpet or the dung of sheep or hares. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | May 8, 2013 |
| Sequencing Facility | University of Pennsylvania DNA Sequencing Facility |
| Shotgun Sequencing Method | 454 Pyrosequencing |
| Approximate Shotgun Coverage | 78 |
| Genome length (bp) | 53008 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGATGGTAA |
| GC Content | 63.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Treddle makes small (1mm and 0.5mm) plaques that are always clear. |
| Morphotype | Siphoviridae |
| Number of Genes | 94 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KP027203 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
3 |
| Pitt Freezer Box Grid# |
B10 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
