| Detailed Information for Phage YoSam321 |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Amber Thurman |
| Year Found | 2016 |
| Location Found | Eubank, KY USA |
| Finding Institution | Somerset Community College |
| Program | Kentucky Biomedical Infrastructure Network Small Genomes Discovery Program |
| From enriched soil sample? | Unknown |
| Isolation Temperature | Not entered |
| GPS Coordinates | 37.3 N, 84.6 W Map |
| Discovery Notes | Large grass side yard that is mainly used by dogs
Some shade during the day
-10.5 degrees Celsius
7 inches |
| Naming Notes | weak headed when sent to TEM (Yosemite Sam (character) was always portrayed as hot-headed, easily to lose temper. 321 represents the countdown to “blast off) |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jul 12, 2022 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 292 |
| Genome length (bp) | 51370 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGCCGGTAA |
| GC Content | 63.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A4 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Somerset Community College |
| Annotation Status | In GenBank |
| Plaque Notes | Clear, fully circular plaques |
| Morphotype | Siphoviridae |
| Number of Genes | 89 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OP820471 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
8.0x10^12 pfu/mL |
| Date of SEA Lysate Titering |
Apr 7, 2016 |
| Pitt Freezer Box# |
28 |
| Pitt Freezer Box Grid# |
B6 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
