| Detailed Information for Phage Zolita |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Samantha Otalvaro |
| Year Found | 2018 |
| Location Found | Providence, RI USA |
| Finding Institution | Providence College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 41.843056 N, 71.438611 W Map |
| Discovery Notes | The soil collected was discovered and retrieved next to a sprinkler. The soil was wet, clumpy, and slightly runny. It was a sunny day with slight clouds. It was warm and 83°F. |
| Naming Notes | "Zola" was the original name for my phage and I got the inspiration for its name from Grey's Anatomy, however, Zola was taken. I opted for Zolita instead. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 3, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1523 |
| Genome length (bp) | 51182 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGGAGGTAA |
| GC Content | 60.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A5 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Providence College |
| Annotation Status | In GenBank |
| Morphotype | Siphoviridae |
| Number of Genes | 85 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN096372 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2.3 x 10^14 |
| Pitt Freezer Box# |
89 |
| Pitt Freezer Box Grid# |
E4 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
