| Detailed Information for Phage Avle17 |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Ann Le |
| Year Found | 2017 |
| Location Found | Charlton, MA USA |
| Finding Institution | Worcester Polytechnic Institute |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 42.120573 N, 71.98548 W Map |
| Discovery Notes | The sample was taken on September 10, 2017 at 9:15 am. The site was chosen on the edge of the flowerbed that has had lots of plant growth and soil amenities added to it over the last 5 years. The general location where the soil was taken is a leaf mulch pile behind 15 T. Hall Road. The type of soil is a loamy soil from the top few centimeters of the flowerbed. The air was about 70 degrees C and the temperature of the soil was about 62 degrees C. A garden trowel was used to remove the loose leaf material over the site and then a hole ~ 3 centimeters deep was created to get to material that was soil like. A plastic sandwich size ziplock bag was filled with the sample. This sample was transported to the lab in Goddard and stored at RT until use. |
| Naming Notes | The name for the phage came from the initials of my own name and the year of the isolation. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 12, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1217 |
| Genome length (bp) | 51366 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGCCGGTAA |
| GC Content | 63.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A4 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Worcester Polytechnic Institute |
| Annotation Status | In GenBank |
| Plaque Notes | The plaque is circular and has a cloudy, turbid morphology. |
| Morphotype | Siphoviridae |
| Number of Genes | 88 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH509443 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.7 X10^10 |
| Pitt Freezer Box# |
66 |
| Pitt Freezer Box Grid# |
D8 |
| Available Files |
| EM Picture | Download |
| GenBank File for Phamerator | Download |