| Detailed Information for Phage Bigfoot |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | James Volz |
| Year Found | 2012 |
| Location Found | Spokane, WA USA |
| Finding Institution | Washington State University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 47.748425 N, 117.425464 W Map |
| Discovery Notes | Found in a fertilized garden, that gets about 8 hrs of sun on average, and is watered daily. Very fertile soil. |
| Naming Notes | My plaques are big, and Bigfoot just seemed like a fitting name |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 19, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 981 |
| Genome length (bp) | 51646 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGATGGTAA |
| GC Content | 63.7% |
| Sequencing Notes | There was a large deletion in some of the phages in the population that was sequenced. In about 47% of reads, there was a 3926 bp deletion from positions 26082 to 30007, inclusive. |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | My plaques consistently exhibited a bulls-eye morphology. They were 7mm across with a 5mm turbid center. |
| Morphotype | Siphoviridae |
| Number of Genes | 87 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KX702320 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
4.0 x 10^10 |
| Date of SEA Lysate Titering |
Nov 13, 2012 |
| Pitt Freezer Box# |
42 |
| Pitt Freezer Box Grid# |
35 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
