Mycobacterium phage Eidsmoe
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Detailed Information for Phage Eidsmoe
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found BySpencer Eidsmoe
Year Found2014
Location FoundBloomington, IL USA
Finding InstitutionIllinois Wesleyan University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates40.461667 N, 88.944167 W Map
Discovery NotesMy bacteriophage was found in a creek. The sample was half silt from the creek bottom and half creek water.
Naming NotesThe name for my phage is my last name. Since my last name is unique I thought it would be suitable for my phage.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 5, 2015
Sequencing FacilityNC State Genomic Sciences Laboratory
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage396
Genome length (bp)52946
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGTCGGTTA
GC Content62.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterA
SubclusterA9
Cluster Life CycleTemperate
Other Cluster Members

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Chargerpower (None)

Abbyshoes (A1)

Abrogate (A1)

Acme (A1)

Adahisdi (A1)

Aeneas (A1)

AFIS (A1)

Agaliana (A1)

Ajay (A1)

Alsfro (A1)

Altman (A1)

Alvin (A1)

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Ciao (A1)

ConceptII (A1)

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DarthPhader (A12)

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Phlei (A13)

Gail (A14)

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Anaysia (A15)

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KatherineG (A15)

LastResort (A15)

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ReMo (A15)

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ShayRa (A15)

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Strosahl (A15)

Switzerland (A15)

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EagleEye (A16)

Lucyedi (A16)

PainterBoy (A16)

40AC (A17)

MyraDee (A18)

Kimona (A19)

20ES (A2)

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Acolyte (A2)

Adzzy (A2)

Agape74 (A2)

AN3 (A2)

AN9 (A2)

ANI8 (A2)

AnnaL29 (A2)

Anselm (A2)

ArcherNM (A2)

BabyBack (A2)

Bactobuster (A2)

Baehexic (A2)

BengiVuitton (A2)

Benvolio (A2)

BiancaTri92 (A2)

BobSwaget (A2)

Bradman (A2)

Bugsy (A2)

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Centaur (A2)

Changeling (A2)

Che12 (A2)

ChipMunk (A2)

Colin (A2)

CRB1 (A2)

Crucio (A2)

D29 (A2)

D32 (A2)

Dalmatian (A2)

DBQu4n (A2)

Deloris (A2)

Dignity (A2)

Drake55 (A2)

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Duplo (A2)

Echild (A2)

Equemioh13 (A2)

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EZMoney23 (A2)

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Wiks (A6)

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Yokurt (A6)

Zaka (A6)

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DroogsArmy (A7)

FlyCatcher (A7)

HINdeR (A7)

Sheen (A7)

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Groundhog (A8)

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Phillis (A8)

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Saintus (A8)

Smeadley (A8)

Stephig9 (A8)

Aliter (A9)

Alma (A9)

Arissanae (A9)

Beemo (A9)

BogosyJay (A9)

Catalina (A9)

Charm (A9)

Conquerage (A9)

Darrell (A9)

DreamTeam1 (A9)

EdogawaKiddo (A9)

Eidsmoe (A9)

Elephantoon (A9)

EmyBug (A9)

ExplosioNervosa (A9)

Fayely (A9)

Hanray (A9)

Holex (A9)

HortumSL17 (A9)

Jiawan (A9)

Keziacharles14 (A9)

Lilleskat (A9)

LoneWolf (A9)

Maminiaina (A9)

Myxus (A9)

Onglai (A9)

PackMan (A9)

Phaeder (A9)

Phonnegut (A9)

Pioneer (A9)

Priya (A9)

Qobbit (A9)

Rahalelujah (A9)

RyeScarlet (A9)

Sachima (A9)

Scherzo (A9)

SheaKeira (A9)

Spouty (A9)

Toaka (A9)

Tubs (A9)

Ugenie5 (A9)

Vanisoa (A9)

Yecey3 (A9)

Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesMy phage had an interesting morphology. I think that my phage is able to switch between the Temperate and Lytic Cycle's. The really cool thing is that I believe that my phage is density dependent with regard to plaque size. When there is a high concentration of pfu on a plate then the plaques appear very small. But when the concentration is less, the plaques tend to be MUCH bigger. I'm not sure if this is a known characteristic of plaques, since I didn't read anything about it in the manual, but this is my theory about why my phage changes plaque size so often.
MorphotypeSiphoviridae
Number of Genes96
Number of tRNAs1
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Eidsmoe_1

Eidsmoe_2

Eidsmoe_3

Eidsmoe_4
(HNH endonuclease)

Eidsmoe_5
(terminase small subunit)

Eidsmoe_6

Eidsmoe_7

Eidsmoe_8

Eidsmoe_9 — tRNA
(tRNA-Glu (ctc))

Eidsmoe_10

Eidsmoe_11
(lysin A)

Eidsmoe_12
(holin)

Eidsmoe_13
(terminase large subunit)

Eidsmoe_14
(portal protein)

Eidsmoe_15
(capsid maturation protease)

Eidsmoe_16
(scaffolding protein)

Eidsmoe_17
(major capsid protein)

Eidsmoe_18
(head-to-tail connector protein)

Eidsmoe_19
(head-to-tail adaptor)

Eidsmoe_20
(head-to-tail connector protein)

Eidsmoe_21
(head-to-tail stopper)

Eidsmoe_22

Eidsmoe_23
(tail terminator)

Eidsmoe_24
(major tail protein)

Eidsmoe_25
(tail assembly chaperone)

Eidsmoe_26
(tail assembly chaperone)

Eidsmoe_27
(tape measure protein)

Eidsmoe_28
(minor tail protein)

Eidsmoe_29

Eidsmoe_30
(minor tail protein)

Eidsmoe_31

Eidsmoe_32
(minor tail protein)

Eidsmoe_33
(minor tail protein)

Eidsmoe_34

Eidsmoe_35

Eidsmoe_36
(ParA-like dsDNA partitioning protein)

Eidsmoe_37
(ParB-like dsDNA partitioning protein)

Eidsmoe_38

Eidsmoe_39

Eidsmoe_40

Eidsmoe_41

Eidsmoe_42

Eidsmoe_43

Eidsmoe_44

Eidsmoe_45
(DNA polymerase I)

Eidsmoe_46

Eidsmoe_47
(HTH DNA binding protein)

Eidsmoe_48

Eidsmoe_49
(ThyX-like thymidylate synthase)

Eidsmoe_50

Eidsmoe_51
(ribonucleotide reductase)

Eidsmoe_52

Eidsmoe_53

Eidsmoe_54

Eidsmoe_55
(RNA polymerase sigma factor)

Eidsmoe_56
(metallophosphoesterase)

Eidsmoe_57

Eidsmoe_58
(NrdH-like glutaredoxin)

Eidsmoe_59
(DNA primase)

Eidsmoe_60
(DNA primase)

Eidsmoe_61

Eidsmoe_62
(endonuclease VII)

Eidsmoe_63

Eidsmoe_64

Eidsmoe_65

Eidsmoe_66

Eidsmoe_67

Eidsmoe_68

Eidsmoe_69
(DnaB-like dsDNA helicase)

Eidsmoe_70

Eidsmoe_71
(helix-turn-helix DNA-binding domain protein)

Eidsmoe_72

Eidsmoe_73
(Cas4 exonuclease)

Eidsmoe_74

Eidsmoe_75
(immunity repressor)

Eidsmoe_76

Eidsmoe_77

Eidsmoe_78

Eidsmoe_79

Eidsmoe_80

Eidsmoe_81

Eidsmoe_82
(DNA methylase)

Eidsmoe_83
(DNA methylase)

Eidsmoe_84
(ParB-like nuclease domain protein)

Eidsmoe_85

Eidsmoe_86

Eidsmoe_87

Eidsmoe_88

Eidsmoe_89

Eidsmoe_90

Eidsmoe_91

Eidsmoe_92

Eidsmoe_93

Eidsmoe_94

Eidsmoe_95

Eidsmoe_96

Eidsmoe_97

Submitted Annotation in DNA Master FormatDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKU716094
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.8*10^11
Pitt Freezer Box# 10
Pitt Freezer Box Grid# F3
Available Files
Final DNAMaster FileDownload