| Detailed Information for Phage Funston |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Frankie Jeney |
| Year Found | 2012 |
| Location Found | Hartford, CT USA |
| Finding Institution | Trinity College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | Not entered |
| GPS Coordinates | 41.744617 N, 72.691836 W Map |
| Discovery Notes | Phage was collected in soil found in a grassy area. This area was near stone and cement. The sample was collected 3/4 inch in deep in the soil. The soil was rather dry. |
| Naming Notes | Named "Funston" because it was found next to a dormitory named Funston. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 13, 2013 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 9249 |
| Genome length (bp) | 51372 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGCCGGTAA |
| GC Content | 64.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A4 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | After 24 hours the plaques were all clear and ranged in diameter from 0.5mm- 3mm. The plaques were also circular in shape. After 48 hours each plaque was had a ring of turbid growth and a clear center. They plaques grew and became amorphous in shape ranging from 1mm-6mm in diameter. |
| Morphotype | Siphoviridae |
| Number of Genes | 88 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KY204247 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
39 |
| Pitt Freezer Box Grid# |
69 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
