| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Noah Marion Crews |
| Year Found | 2015 |
| Location Found | Bowling Green, KY USA |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 36.963333 N, 86.368333 W Map |
| Discovery Notes | Found in the backyard of our family home. The phages were taken from soil directly under a compost pile that runs adjacent to a forest and a creek. There was about a cm of wet mud, and underneath was solid, tough earth. A fair medley of muck and good earth were taken. |
| Naming Notes | The naming of the Phage is based on a popular character in many Role Playing Games. The Barbarian Orc, Gruunaga. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 5, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 530 |
| Genome length (bp) | 52195 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGTCGGTAA |
| GC Content | 61.4% |
| Sequencing Notes | There is a polymorphism in the population. At position 23564, about 60% of the reads had the "G" that's in the final consensus sequence, while the other 40% had an "A". |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A6 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | Central region is lysed while a turbid layer rings about the plaques |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | The plaque is of the bull's eye variant. Where the center is lysed and the edges of the colony are turbid. It generally needs between a 24-48 hour window to grow at 30 Celsius, and the plaques themselves are large and easy enough to see. |
| Morphotype | Siphoviridae |
| Number of Genes | 101 |
| Number of tRNAs | 3 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KX576638 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
Gruunaga |
| SEA Lysate Titer |
4.0 x 10^10 |
| Date of SEA Lysate Titering |
Nov 9, 2015 |
| Pitt Freezer Box# |
27 |
| Pitt Freezer Box Grid# |
E7 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
