| Detailed Information for Phage Gyzlar |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Jamila Shah |
| Year Found | 2020 |
| Location Found | Warren, NJ United States |
| Finding Institution | Lehigh University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 6°C |
| GPS Coordinates | 40.624964 N, 74.560036 W Map |
| Discovery Notes | -The soil was wet and full of organic matter.
-It was collected approximately 2 inches below the surface.
-The ambient temperature was approximately 5.6 degrees C.
The phage was adopted from Nandini Yellamelli. |
| Naming Notes | Gyzlar means 'girls' in my language (Turkmen). I have 3 sisters and I love them very much. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 19, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 2784 |
| Genome length (bp) | 49064 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGATGGTAA |
| GC Content | 63.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Lehigh University |
| Annotation Status | In GenBank |
| Plaque Notes | 24-hour observation:
-Plaques were approximately 3-6 mm in diameter. Only some of the plaques showed a cloudy ring around.
48-hour observation:
-Plaque size ranged from 3-6 mm.
-All the plaques showed a cloudy ring around. Some of the larger ones also had a smaller ring within the plaque. Besides that, all the plaques had clear centers. |
| Morphotype | Siphoviridae |
| Number of Genes | 87 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MZ355726 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
124 |
| Pitt Freezer Box Grid# |
G9 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| GenBank File for Phamerator | Download |
