| Detailed Information for Phage Iceman |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Jace Bradshaw and Laura Prince |
| Year Found | 2013 |
| Location Found | Arkadelphia, AR USA |
| Finding Institution | Ouachita Baptist University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 34.118782 N, 93.096006 W Map |
| Discovery Notes | Our soil sample was taken approximately 4 centimeters deep. It was collected at 6:50 p.m. on August 25, 2013. The temperature was 87 degrees Fahrenheit with a relative humidity of 74%. The soil was very dry and the land was always in the sun. The grade was nearly flat because the soil was recently cleared and leveled. |
| Naming Notes | The name came from a mutual love for the movie Top Gun. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 15, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 3689 |
| Genome length (bp) | 51662 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGCCGGTAA |
| GC Content | 63.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A4 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Ouachita Baptist University |
| Annotation Status | In GenBank |
| Plaque Notes | We observed pin-prick size to 2 millimeter sized plaques. The turbidity varied: we had some plaques that had clear centers and cloudy edges and some that were completely cloudy throughout. There was no apparent trend in the correlation with relation to size or ratio from plate to plate. |
| Morphotype | Siphoviridae |
| Number of Genes | 86 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | ON970565 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
4.9x10(^8) pfu/mL |
| Date of SEA Lysate Titering |
Oct 21, 2013 |
| Pitt Freezer Box# |
2 |
| Pitt Freezer Box Grid# |
E5 |
| Available Files |
| Plaque Picture | Download |
| GenBank File for Phamerator | Download |
