| Detailed Information for Phage Jeeves |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Jon Reid |
| Year Found | 2016 |
| Location Found | Olympia, WA USA |
| Finding Institution | Gonzaga University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 25°C |
| GPS Coordinates | 47.076319 N, 122.829517 W Map |
| Discovery Notes | Jeeves was found in a soil sample taken from a vegetable garden. It was 1 degree Celsius when the sample was collected. It was relatively damp and cloudy outside as well. |
| Naming Notes | The name comes from the fictional character Reginald Jeeves, a character created by author P.G. Wodehouse. The name is thought of as the quintessential butler or valet name. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 16, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1444 |
| Genome length (bp) | 52466 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGTCGGTTA |
| GC Content | 62.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A14 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | Since the plaques formed by Jeeves were turbid, Jeeves displayed characteristics of a phage following a temperate life-cycle. |
| Other Cluster Members |
|
| Annotating Institution | Gonzaga University |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques formed by Jeeves were turbid and averaged 6 mm in diameter. Along with their turbid appearance, a secondary halo-like ring formed just inside of the plaque edge. |
| Morphotype | Siphoviridae |
| Number of Genes | 101 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN310541 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
40 |
| Pitt Freezer Box Grid# |
G11 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
