Mycobacterium phage KittenMittens
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| Detailed Information for Phage KittenMittens | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Kristofer Miller |
| Year Found | 2012 |
| Location Found | Hampden Sydney, VA USA |
| Finding Institution | Hampden-Sydney College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 37.243500 N, 78.454000 W Map |
| Discovery Notes | The soil sample was taken from an area beside the Lagoon Athletic Fields on the campus of Hampden-Sydney College. |
| Naming Notes | The name comes from an episode of "It's Always Sunny in Philadelphia." |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 5, 2013 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 560 |
| Genome length (bp) | 49894 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGCCGGTAA |
| GC Content | 64.5% |
| Sequencing Notes | This phage has a polymorphism at position 22909. The consensus at this position is a C, but 40% of the reads had a T in this position. |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | A |
| Subcluster | A10 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members | |
| Annotating Institution | Hampden-Sydney College |
| Annotation Status | In GenBank |
| Morphotype | Siphoviridae |
| Number of Genes | 78 |
| Number of tRNAs | 3 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List | |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH001459 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 36 |
| Pitt Freezer Box Grid# | 30 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |