Mycobacterium phage LunarLander
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Detailed Information for Phage LunarLander
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Duplicates of this PhageBrightBird
Found ByLuke Aydelotte
Year Found2024
Location FoundOrem, UT United States of America
Finding InstitutionBrigham Young University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature28°C
GPS Coordinates40.310672 N, 111.708419 W Map
Naming NotesElectron microscopy imaging provided definitive evidence that the isolated phage belongs to the Siphoviridae family, characterized by their non-enveloped, icosahedral heads and long, non-contractile tails. While the accompanying electron micrograph doesn't offer the highest resolution, careful observation reveals subtle yet distinctive tail fibers extending from the distal end of the phage's sheath. These fibers, reminiscent of the landing struts on a lunar module, create a striking visual analogy, with the phage poised as if ready to gently alight. However, the phage's destination is not the lunar surface but the bacterial cell wall of Mycobacterium smegmatis mc^2 155. The phrase "our little phage phriends" is used endearingly to emphasize a personal connection to the discovery and investigation of this novel bacteriophage.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 10, 2025
Sequencing FacilityCD Genomics
Shotgun Sequencing MethodIllumina
Sequencer UsedIllumina NovaSeq X Plus
Read TypePaired-end reads
Read Length150 bp
Approximate Shotgun Coverage6590
Genome length (bp)48971
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGATGGTAA
GC Content64.2%
Fasta file available?Yes: Download fasta file
Characterization
ClusterA
SubclusterA1
Cluster Life CycleTemperate
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Edison31 (A10)

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Kristoff (A10)

OKCentral2016 (A10)

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Rebeuca (A10)

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Rowdy (A10)

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WalterMcMickey (A10)

Aneem (A11)

Bachome (A11)

Bowtie (A11)

Bud (A11)

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Et2Brutus (A11)

Fibonacci (A11)

Flaverint (A11)

Gilberta (A11)

Hutc2 (A11)

Insomnia (A11)

Jabith (A11)

Joselito (A11)

Kimba (A11)

Lucivia (A11)

Mabel (A11)

MaCh (A11)

Mulciber (A11)

Munch (A11)

Orange (A11)

Petersenfast (A11)

Salz (A11)

Sham4 (A11)

Snape (A11)

Timothy (A11)

TinyTimmy (A11)

Wolpertinger (A11)

DarthPhader (A12)

Pharaoh (A12)

Refuge (A12)

Steamy (A12)

Zimmer (A12)

Phlei (A13)

Gail (A14)

Jeeves (A14)

Luchador (A14)

SororFago (A14)

Sunhee (A14)

Anaysia (A15)

Anon (A15)

Battleship (A15)

Boohoo (A15)

DekHockey33 (A15)

Epsocamisio (A15)

JSwag (A15)

KatherineG (A15)

LastResort (A15)

Looper (A15)

MinecraftSteve (A15)

Nebulosus (A15)

Nedarya (A15)

Oofda (A15)

ReMo (A15)

Remus (A15)

Rosalind (A15)

ShayRa (A15)

Soups (A15)

Strosahl (A15)

Switzerland (A15)

Waits (A15)

Warrior24 (A15)

EagleEye (A16)

Lucyedi (A16)

PainterBoy (A16)

40AC (A17)

MyraDee (A18)

Kimona (A19)

20ES (A2)

AbbyPaige (A2)

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Adzzy (A2)

Agape74 (A2)

AN3 (A2)

AN9 (A2)

ANI8 (A2)

AnnaL29 (A2)

Anselm (A2)

ArcherNM (A2)

BabyBack (A2)

Bactobuster (A2)

Baehexic (A2)

BengiVuitton (A2)

Benvolio (A2)

BiancaTri92 (A2)

BobSwaget (A2)

Bradman (A2)

Bugsy (A2)

Caraxes (A2)

Centaur (A2)

Changeling (A2)

Che12 (A2)

ChipMunk (A2)

Colin (A2)

CRB1 (A2)

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D29 (A2)

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DeerParkTS (A2)

Deloris (A2)

Dignity (A2)

Drake55 (A2)

DudeLittle (A2)

Duplo (A2)

Echild (A2)

Equemioh13 (A2)

EvilGenius (A2)

EZMoney23 (A2)

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FiringLine (A2)

First (A2)

Flare16 (A2)

ForesterFrank (A2)

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Georgie2 (A2)

Gratitude (A2)

HarryHoudini (A2)

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Herbertwm (A2)

IronMan (A2)

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JoshKayV (A2)

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NothingSpecial (A2)

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Power (A2)

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Rachaly (A2)

RedRock (A2)

Retro23 (A2)

SemperFi (A2)

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Sknot (A2)

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StarStuff (A2)

Superchunk (A2)

SweetiePie (A2)

SwirlSquare (A2)

TarsusIV (A2)

Terrific (A2)

TipsytheTRex (A2)

Tomathan (A2)

Travvers (A2)

Tristan (A2)

Trixie (A2)

Trooper (A2)

Turbido (A2)

Updawg (A2)

VA6 (A2)

VC3 (A2)

WeiHuaDA (A2)

Whabigail7 (A2)

WideWale (A2)

XianYue (A2)

Anthony (A20)

FatCactus (A20)

Kinmap (A21)

Chargerpower (A22)

ACFishhook (A3)

Aglet (A3)

AgronaGT15 (A3)

Anubis (A3)

AugsMagnumOpus (A3)

BabyJohn (A3)

BabyRay (A3)

Beauxregard13 (A3)

Bigflo (A3)

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BreSam8 (A3)

Bugatti (A3)

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Calvinny (A3)

Caviar (A3)

Chiqui (A3)

Clammy (A3)

Colbster (A3)

Cullens (A3)

DaHudson (A3)

Daishi (A3)

Dieselweasel (A3)

DropBear (A3)

EpicPhail (A3)

Farber (A3)

Fernando (A3)

Fred313 (A3)

Gemma (A3)

GingkoMaracino (A3)

Giroux (A3)

Grif (A3)

Groupthink (A3)

Grub (A3)

Grum1 (A3)

GtownJaz (A3)

Gwennie (A3)

HashRod (A3)

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Kalnoky (A3)

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LBerry (A3)

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Louie6 (A3)

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MadMarie (A3)

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Relief (A4)

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Rosa24 (A4)

Ruin (A4)

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Thanksgivukkah (A4)

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AgentM (A5)

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Maminiaina (A9)

Myxus (A9)

Onglai (A9)

PackMan (A9)

Phaeder (A9)

Phonnegut (A9)

Pioneer (A9)

Priya (A9)

Qobbit (A9)

Rahalelujah (A9)

RyeScarlet (A9)

Sachima (A9)

Scherzo (A9)

SheaKeira (A9)

Spouty (A9)

Toaka (A9)

Tubs (A9)

Ugenie5 (A9)

Vanisoa (A9)

WorldCup (A9)

Yecey3 (A9)

Annotating InstitutionBrigham Young University
Annotation StatusIn GenBank
MorphotypeSiphoviridae
Number of Genes80
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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LunarLander_1
(HNH endonuclease)

LunarLander_2

LunarLander_3
(terminase, small subunit)

LunarLander_4

LunarLander_5
(tail collar fibers protein)

LunarLander_6

LunarLander_7
(tail collar spacer)

LunarLander_8

LunarLander_9
(lysin A)

LunarLander_10
(lysin B)

LunarLander_11
(terminase, large subunit)

LunarLander_12
(portal protein)

LunarLander_13
(capsid maturation protease)

LunarLander_14
(scaffolding protein)

LunarLander_15
(major capsid protein)

LunarLander_16

LunarLander_17
(head-to-tail adaptor)

LunarLander_18
(head-to-tail stopper)

LunarLander_19

LunarLander_20
(tail terminator)

LunarLander_21
(tail tube)

LunarLander_22
(tail assembly chaperone)

LunarLander_23
(tail assembly chaperone)

LunarLander_24
(tape measure protein)

LunarLander_25
(tail tip cage/Dit)

LunarLander_26

LunarLander_27
(baseplate hub)

LunarLander_28

LunarLander_29

LunarLander_30

LunarLander_31

LunarLander_32
(tail spike)

LunarLander_33
(tail wing base/D-alanyl-D-alanine carboxypeptidase)

LunarLander_34
(tail wing arm)

LunarLander_35

LunarLander_36
(tail wing brush)

LunarLander_37

LunarLander_38
(serine integrase)

LunarLander_39

LunarLander_40

LunarLander_41

LunarLander_42

LunarLander_43

LunarLander_44

LunarLander_45

LunarLander_46
(DNA polymerase I)

LunarLander_47

LunarLander_48

LunarLander_49

LunarLander_50

LunarLander_51
(DNA binding protein)

LunarLander_52

LunarLander_53
(recombination directionality factor)

LunarLander_54

LunarLander_55

LunarLander_56
(DNA primase)

LunarLander_57
(DNA primase)

LunarLander_58

LunarLander_59

LunarLander_60
(DNA methyltransferase)

LunarLander_61
(endonuclease VII)

LunarLander_62
(NrdH-like glutaredoxin)

LunarLander_63
(DnaB-like dsDNA helicase)

LunarLander_64

LunarLander_65

LunarLander_66

LunarLander_67
(helix-turn-helix DNA binding domain)

LunarLander_68
(Cas4 family exonuclease)

LunarLander_69

LunarLander_70

LunarLander_71

LunarLander_72

LunarLander_73

LunarLander_74

LunarLander_75

LunarLander_76

LunarLander_77

LunarLander_78

LunarLander_79

LunarLander_80

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionPX136951
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 209
Pitt Freezer Box Grid# A8
Available Files
EM PictureDownload
GenBank File for PhameratorDownload