Mycobacterium phage Marsha
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| Detailed Information for Phage Marsha | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Danielle Goodman |
| Year Found | 2009 |
| Location Found | Holland, MI US |
| Finding Institution | Hope College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 42.793995 N, 86.098732 W Map |
| Discovery Notes | Trees, small plants (i.e. ferns, weeds), edge of forest, about 3 feet in. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 5, 2020 |
| Sequencing Facility | Hope College |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 104 |
| Genome length (bp) | 54459 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGATGGTAA |
| GC Content | 63.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | A |
| Subcluster | A1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members | |
| Annotating Institution | University of California, Los Angeles |
| Annotation Status | In GenBank |
| Plaque Notes | 2 – 5 mm, clear center with defined edges and a turbid ring around it, incubated for 24 hours at 37ºC |
| Morphotype | Siphoviridae |
| Number of Genes | 98 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List | |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MW862983 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 2.4e10 |
| Pitt Freezer Box# | 4 |
| Pitt Freezer Box Grid# | 3 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
