| Detailed Information for Phage McGuire |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Shea Dolan |
| Year Found | 2013 |
| Location Found | New Haven, CT USA |
| Finding Institution | Southern Connecticut State University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 42°C |
| GPS Coordinates | 41.334444 N, 72.942778 W Map |
| Discovery Notes | This plaque was discovered in moist soil under a compost pile. It approximately 63 degree F and the soil sample taken was about 3.6 cm in the soil. The soil was sheltered from the sunlight because of trees and leaves. This soil sample was about 2 feet from a dumpster. Surrounding this area is mostly soil with little grass. The mean capsid width is 67.61 nm with a standard deviation of 2.56 nm. The mean tail length is 159.49 nm with a standard deviation of 8.405. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 27, 2014 |
| Sequencing Facility | Virginia Commonwealth University Nucleic Acids Research Facilities |
| Shotgun Sequencing Method | 454 |
| Sequencer Used | 454 Genome Sequencer FLX |
| Approximate Shotgun Coverage | 670 |
| Sanger Finishing Reactions | 2 |
| Genome length (bp) | 51521 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGATGGTAA |
| GC Content | 63.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | This phage is temperate and large. The diameter ranges between 1 and 6 mm although it is most usually around 4.5 mm. |
| Morphotype | Siphoviridae |
| Number of Genes | 92 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MG962372 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
6.8x10^9 |
| Date of SEA Lysate Titering |
Oct 22, 2013 |
| Pitt Freezer Box# |
10 |
| Pitt Freezer Box Grid# |
B2 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
