| Detailed Information for Phage OKaNui |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Kaitlyn Mayfield |
| Year Found | 2018 |
| Location Found | Meridian, MS USA |
| Finding Institution | University of West Alabama |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 32.587275 N, 88.187355 W Map |
| Discovery Notes | I found it in a trough that was formed under a window. The trough was caused by rain runoff from a building. Soil sample was slightly moist and was collected and refrigerated for preservation. It was found using enriched isolation. |
| Naming Notes | The name sources from the Hawaiian name of O' Ka Nui. This means Phage the Great. The phage was named this because I wanted a name that communicated it aggressive manner of eating a lot of bacteria. It created larger clearings in the bacteria surrounding it. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 26, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 874 |
| Genome length (bp) | 51424 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGCCGGTAA |
| GC Content | 63.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A4 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of West Alabama |
| Annotation Status | In GenBank |
| Plaque Notes | It creates relatively large spots. It is easily visible to the naked eye. When diluted, the phage is very easily seen and counted. (It will clear entire plates if left too incubate too long.) |
| Morphotype | Siphoviridae |
| Number of Genes | 87 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT490373 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
85 |
| Pitt Freezer Box Grid# |
E11 |
| Available Files |
| GenBank File for Phamerator | Download |