| Detailed Information for Phage Penny1 |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Nicole Cullen |
| Year Found | 2014 |
| Location Found | Providence, RI USA |
| Finding Institution | Providence College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 41.8433 N, 71.4346 W Map |
| Discovery Notes | Soil sample taken on May 27 at 2:30pm. Phage obtained from soil sample taken from dry soil 2" deep under a tree in front of Albertus Magnus Hall. The weather was 72 degrees Fahrenheit, cloudy and 64% humidity. |
| Naming Notes | Penny named after character from the show Big Bang Theory. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 6, 2015 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 2638 |
| Genome length (bp) | 50884 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGGTGGTAA |
| GC Content | 64.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A3 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Forms circular plaques about 3.5nm in diameter.
|
| Morphotype | Siphoviridae |
| Number of Genes | 90 |
| Number of tRNAs | 3 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KX458237 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.5 x 10^9 pfu/mL |
| Date of SEA Lysate Titering |
Jun 26, 2014 |
| Pitt Freezer Box# |
89 |
| Pitt Freezer Box Grid# |
E7 |
| Available Files |
| Plaque Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |