| Detailed Information for Phage Pharaoh |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Allison Lindquist |
| Year Found | 2015 |
| Location Found | Louisville, CO USA |
| Finding Institution | Hope College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 32°C |
| GPS Coordinates | 38.99 N, 105.15 W Map |
| Discovery Notes | The soil sample was collected in urban soil that was relatively isolated from cement, asphalt etc. It was in an un-landscaped corner of a backyard under approx. 3 cm of decaying leaves and pine needles. |
| Naming Notes | This phage produced extremely turbid plaques... meaning each plate infection was much like searching for buried treasure. Thus, this phage is the "king" of the treasure and is named after ancient Egyptian kings. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 18, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 3384 |
| Genome length (bp) | 50346 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGGACGTAA |
| GC Content | 63.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A12 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Marywood University |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are extremely turbid, spherical in shape and quite small. The plaque diameter ranges from 2 mm in size to 3 mm in size. |
| Morphotype | Siphoviridae |
| Number of Genes | 80 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK524530 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
9.9 x 10^8 pfu |
| Pitt Freezer Box# |
19 |
| Pitt Freezer Box Grid# |
E3 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
