| Detailed Information for Phage Prudy |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Sonto Dube |
| Year Found | 2025 |
| Location Found | Cape Town, South Africa |
| Finding Institution | TASK South Africa |
| Program | Unavailable |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 33.954809 S, 18.461273 E Map |
| Discovery Notes | Soil was collected from a grassy, humid area about 5 cm deep and stored in an airtight specimen jar. It was enriched with Mycobacterium smegmatis and incubated overnight at 37°C to promote phage amplification. |
| Naming Notes | The phage is named in memory of my beloved late cousin, Prudence. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 11, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 2874 |
| Genome length (bp) | 52746 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGTCGGTTA |
| GC Content | 64.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A2 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | TASK South Africa |
| Annotation Status | Being Annotated (Expected completion by 2/11/2026) |
| Has been Phamerated? | No |
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
